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MR-070

EmbryoMax® Human Tubal Fluid (HTF) (1X), liquid, for Mouse IVF

The EmbryoMax Human Tubal Fluid (HTF) (1X), liquid designed for use with Mouse IVF is available in a 50 mL format and has been optimized and validated for Embryo Culture.

Synonyme(s) :

HTF Media, Human Tubul Fluid Media

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A propos de cet article

UNSPSC Code:
12352207
NACRES:
NA.75
eCl@ss:
32160801
Le tarif et la disponibilité ne sont pas disponibles actuellement.
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Quality Segment

300

form

liquid

manufacturer/tradename

Specialty Media, EmbryoMax®

technique(s)

cell culture | embryo: suitable, cell culture | stem cell: suitable

input

sample type: mouse embryo(s)

General description

The EmbryoMax® Human Tubal Fluid (HTF) (1X), liquid designed for use with Mouse IVF is available in a 50 mL format and has been optimized and validated for embryo Culture.

Application

EmbryoMax® Human Tubal Fluid (HTF) (1X), liquid has been used as an in vitro fertilization (IVF) media for mouse oocytes and embryo.

Preparation Note

Can be stored at -20°C upto the expiration date on the bottle.


Once thawed it should be used within 2 weeks

Legal Information

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Cet article
MR-024SCM103SCM130
EmbryoMax® Human Tubal Fluid (HTF) (1X), liquid, for Mouse IVF The EmbryoMax Human Tubal Fluid (HTF) (1X), liquid designed for use with Mouse IVF is available in a 50 mL format and has been optimized and validated for Embryo Culture.

Sigma-Aldrich

MR-070

EmbryoMax® Human Tubal Fluid (HTF) (1X), liquid, for Mouse IVF

EmbryoMax® FHM Mouse Embryo Media (1X), liquid, with Phenol Red

Sigma-Aldrich

MR-024

EmbryoMax® FHM Mouse Embryo Media

EmbryoMax® MEF Conditioned Media EmbryoMax MEF Conditioned Media (MEF-CM) can be used for the feeder-free culture of human ES/iPS cells.

Sigma-Aldrich

SCM103

EmbryoMax® MEF Conditioned Media

Milieu PluriSTEM® pour cellules ES/iPS humaines Defined small molecule based serum-free medium that enables feeder-free culture of human ES/iPS cells and allows for media exchanges every other day without compromising the morphology or long term functionality of human pluripotent stem cells.

Sigma-Aldrich

SCM130

Milieu PluriSTEM® pour cellules ES/iPS humaines

form

liquid

form

liquid

form

-

form

liquid

technique(s)

cell culture | embryo: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | embryo: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | embryo: suitable, cell culture | stem cell: suitable

Quality Level

300

Quality Level

300

Quality Level

100

Quality Level

100

manufacturer/tradename

Specialty Media, EmbryoMax®

manufacturer/tradename

Specialty Media, EmbryoMax®

manufacturer/tradename

Specialty Media, EmbryoMax®

manufacturer/tradename

PluriSTEM®

input

sample type: mouse embryo(s)

input

sample type: mouse embryo(s)

input

-

input

-

Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 2

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Questions

1–5 of 5 Questions  

  1. what is the normal pH range of this product?

    1 answer

    1. This product is supplied at pH range of 7.0 - 7.4. This information is lot-specific and reported in the Certificate of Analysis.

      Helpful?

  2. Once thawed, can the media be aliquoted and refrozen?

    1 answer

    1. For product MR-070, once thawed, this should be used within 2 weeks. This cannot be aliquoted, thawed, and refrozen. This product can be stored at -20°C upto the expiration date on the bottle.

      Helpful?

  3. How long can this be stored at -80C?

    1 answer

    1. This product may be stored at -80°C up to the expiration date on the bottle. However, note that storing this material at -80°C may not significantly increase the shelf life.

      Helpful?

  4. Should we supplement BSA to the HTF for embryo culture or can we use it directly use it ?

    1 answer

    1. The product is already supplemented with 4.0 g/L BSA.

      Helpful?

  5. Hi! I've been trying to find the detailed composition of the media and couldn't find it. Could you please let me know if the media is supplemented with BSA or is it needed? What buffer does it uses? Thanks in advance, Best, Maria

    1 answer

    1. The composition of the medium MR-070-D EmbryoMax® is as follows:

      Component g/L
      BSA 4.0
      CaCl2-2H2O 0.3
      Glucose 0.5
      KCl 0.3496
      KH2PO4 0.0504
      MgSO4-7H2O 0.0493
      NaCl 5.9375
      NaHCO3 2.1
      Sodium Lactate 3.42 mg/L
      Sodium Pyruvate 0.0365
      Penicillin G Na Salt 0.075
      Phenol Red 2mg/L
      Streptomycin Sulfate 0.05

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