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Key Documents

MABN70

Sigma-Aldrich

Anti-Slo1 Antibody, clone L6/60

clone L6/60, from mouse

Synonyme(s) :

potassium large conductance calcium-activated channel, subfamily M, alpha member 1, Slo homolog, stretch-activated Kca channel, Maxi K channel, Drosophila slowpoke-like, Calcium-activated potassium channel, subfamily M subunit alpha-1, Slowpoke homolog,

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

L6/60, monoclonal

Espèces réactives

rat

Réactivité de l'espèce (prédite par homologie)

mouse (immunogen homology)

Technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

Isotype

IgG2aκ

Numéro d'accès NCBI

NP_034740

Numéro d'accès UniProt

Q08460

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... KCNMA1(3778)

Description générale

Slo1 (Calcium-activated potassium channel subunit alpha-1) may not only be activated by membrane depolarization but may also be by an increase in cytosolic Ca2+. The concentration of cytosolic Mg2+ also activates this potassium channel. Found to play a part in the repolarization of the membrane potential due to the fact that it diminishes the excitatory voltage in the cell.

Spécificité

This antibody recognizes S9-S10 segments of Slo1.

Immunogène

Epitope: S9-S10 segments
Recombinant protein corresponding to the S9-S10 segments of mouse Slo1.

Application

Detect Slo1 using this Anti-Slo1 Antibody, clone L6/60 validated for use in IH, WB & IC.
Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Slo1 in rat cerebellum tissue.

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (Misonou, H., et al. (2006). J Comp Neurol. 496(3):289–302.)
Research Category
Neuroscience
Research Sub Category
Signaling Neuroscience

Qualité

Evaluated by Western Blot in rat brain membrane tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Slo1 on 10 µg of rat brain membrane tissue lysate.

Description de la cible

~100 kDa observed

Forme physique

Format: Purified
Protein G
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Rat brain membrane tissue lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Boxing Li et al.
Nature neuroscience, 17(8), 1055-1063 (2014-06-24)
Ion channels are essential for the regulation of neuronal functions. The significance of plasma membrane, mitochondrial, endoplasmic reticulum and lysosomal ion channels in the regulation of Ca(2+) is well established. In contrast, surprisingly little is known about the function of
Andrew J Nickerson et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(5), e21606-e21606 (2021-04-29)
Na+ -K+ -2Cl- cotransporter-1 (NKCC1) facilitates basolateral K+ and Cl- uptake, supporting their efflux across mucosal membranes of colonic epithelial cells. NKCC1 activity has also been shown to be critical for electrogenic K+ secretion induced by aldosterone, which is known
Oscar Vivas et al.
eLife, 6 (2017-07-01)
CaV-channel dependent activation of BK channels is critical for feedback control of both calcium influx and cell excitability. Here we addressed the functional and spatial interaction between BK and CaV1.3 channels, unique CaV1 channels that activate at low voltages. We
Agbonlahor Okhuarobo et al.
Molecular psychiatry (2023-12-23)
Large conductance potassium (BK) channels are among the most sensitive molecular targets of ethanol and genetic variations in the channel-forming α subunit have been nominally associated with alcohol use disorders. However, whether the action of ethanol at BK α influences
Shangru Lyu et al.
Neuroscience, 505, 78-90 (2022-10-17)
Genetic analyses have linked BTBD9 to restless legs syndrome (RLS) and sleep regulation. Btbd9 knockout mice show RLS-like motor restlessness. Previously, we found hyperactivity of cerebellar Purkinje cells (PCs) in Btbd9 knockout mice, which may contribute to the motor restlessness
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