MABN543

Anti-Gs protein Antibody, alpha subunit, clone N192/12

clone N192/12, from mouse

• Synonyme(s) : Guanine nucleotide-binding protein G(s) subunit alpha isoforms short, Adenylate cyclase-stimulating G alpha protein
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: N192/12, monoclonal
• Espèces réactives: rat, human
• Réactivité de l'espèce (prédite par homologie): bovine (immunogen homology)
• Technique(s): western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_851364
• Numéro d'accès UniProt: P04896
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... GNAS(2778)

Description

Description générale

The heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) mediate signaling from a large number of G-protein coupled receptors (GPCR) to a variety of intracellular effectors. G-proteins are composed of a 36-52 kDa α-subunit, a 35-36 kDa β-subunit, and an 8-10 kDa γ-subunit. They are classified based on the nature of their α-subunit. Gs is ubiquitously expressed and couples receptors to adenylyl cyclase and promotes intracellular cAMP generation. Mutations in Gs-α can lead to constitutive activation of receptors leading to development of endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. In brain tissue Gs-α activates adenyl cyclase in direct response to stimulation of several neurotransmitter receptors and facilitates depolarization-induced neurotransmitter release for synaptic transmission. Also, it is shown that Gs-stimulated adenylyl cyclase activity is significantly reduced in the Alzheimer′s disease brain tissue.

Spécificité

Other homologies: Mouse (99% sequence homology).

Immunogène

Recombinant protein corresponding to bovine GS protein, alpha subunit.

Application

Anti-Gs protein Antibody, alpha subunit, clone N192/12 is a highly specific mouse monoclonal antibody & that targets Gsα & has been tested in western blotting.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected GS protein, alpha subunit in 10 µg of SH-SY5Y cell lysate.

Qualité

Evaluated by Western Blotting in rat brain membrane tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected GS protein, alpha subunit in 10 µg of rat brain membrane tissue lysate.

Description de la cible

~45 kDa observed

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Rat brain membrane tissue lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable