MABN2470

Anti-Aquaporin-4 Antibody, clone mCCD

clone mCCD, from mouse

• Synonyme(s) : AQP-4, Mercurial-insensitive water channel, MIWC, WCH4
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: mCCD, monoclonal
• Espèces réactives: human, rat, mouse
• Conditionnement: antibody small pack of 25 μg
• Technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG3κ
• Numéro d'accès NCBI: NP_001641
• Numéro d'accès UniProt: P55087
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... AQP4(361)

Description

Description générale

Aquaporin-4 (UniProt: P55087; also known as AQP-4, Mercurial-insensitive water channel, MIWC, WCH4) is encoded by the AGP4 gene (Gene ID: 361) in human. Aquaporin-4 is a multi-pass membrane protein that forms a water-specific channel and serves as an osmoreceptor that regulates body water balance and mediates water flow within the central nervous system. Its extracellular domains are localized to amino acids 58-64, 137-155, and 206-231); its transmembrane domains are in amino acids 37-57, 65-85, 116-136, 156-176, 185-205, and 232-252); and the cytoplasmic domain is in amino acids 1-36, 86-115, 177-184, and 253-323). Aquaporin-4 is largely expressed in the brain and the skeletal muscle and at lower levels in heart, kidney, and lung tissue. Phosphorylation of Aquaporin-4 at Serine 180 by protein kinase C is shown to reduce conductance by about 50%, whereas phosphorylation at Serine 111 by protein kinase G in response to glutamate can increase conductance by about 40%. Two isoforms of Aquaporin-4 have been described that are produced by alternative splicing. Antibodies to Aquaporin-4 can serve as useful serum markers to distinguishing neuromyelitis optica (NM) from multiple sclerosis. Clone mCCD does not cause redistribution of Aquaporin-4 and also it does not affect coaggregation astrocytic CD32-B and Aquaporin-4. (Ref.: Hinson, SR et al. (2017). Proc. Natl. Acad. Sci. USA 114(21); 5491-5496).

Spécificité

Clone mCCD specifically detects Aquaporin-4 in human, mouse, and rat brain tissue sections. It targets an epitope with in 71 amino acids from the cytoplasmic domain from the C-terminal region.

Immunogène

Epitope: cytoplasmic domain

His-tagged full length human recombinant Aquaporin-4.

Application

Anti-Aquaporin-4, clone mCCD Antibody, Cat. No. MABN2470, is a highly specific mouse monoclonal antibody that targets Aquaporin-4 and has been tested for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A representative lot detected Aquaporin-4 in Western Blotting applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Aquaporin-4 in Immunoprecipitation applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).

Immunocytochemistry Analysis: A representative lot detected Aquaporin-4 in Immunocytochemistry applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).

Qualité

Evaluated by Immunohistochemistry in rat brain frozen tissue sections.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Aquaporin-4 in rat brain frozen tissue sections.

Description de la cible

34.83 kDa calculated.

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG3 in PBS without preservatives.

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable