MABF374

Anti-CD11c Antibody (mouse), FITC, clone N418

clone N418, 0.5 mg/mL, from hamster(Armenian)

• Synonyme(s) : Integrin alpha-Xl, CD11 antigen-like family member C, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor p150,95, CD11c
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.44

Propriétés

• Source biologique: hamster (Armenian)
• Niveau de qualité: 100
• Conjugué: FITC conjugate
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: N418, monoclonal
• Espèces réactives: mouse
• Concentration: 0.5 mg/mL
• Technique(s): flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• Isotype: IgG
• Numéro d'accès UniProt: Q9QXH4
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Itgax(16411)

Description

Description générale

The N418 antibody reacts with mouse CD11c, also known as integrin alpha X. This 150 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha X (CD11c) assembles with Integrin beta-2 (CD18) into a receptor complex known as CR4 which can bind and induce signaling throµgh ICAMs and VCAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.The N418 antibody is widely used as a marker for CD11c expression on dendritic cells (DC), often in parallel with markers for CD11b, for identification of developmental stages and mature subsets of this cell type. CD11c is prominently expressed on tissue macrophages, and is also detected on some types of activated T cells and intestinal intraepithelial lymphocytes (IEL).

Immunogène

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology

This armenian hamster monoclonal antibody Anti-CD11c (mouse), FITC, clone N418 validated for use in IP, IHC, Immunofluorescence & Flow Cytometry for the detection of CD11c.

Qualité

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected CD11c in one million C57Bl/6 splenocytes.

Forme physique

Purified Armenian Hamster Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Size Exclusion Chromatography

Stockage et stabilité

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable