MABF2060

Anti-IL13R alpha 1 Antibody, clone IG3

clone IG3, from mouse

• Synonyme(s) : Interleukin-13 receptor subunit alpha-1, IL-13 receptor subunit alpha-1, IL-13R subunit alpha-1, IL-13RA1, Interleukin-13-binding protein, Novel cytokine receptor 4, NR4, CD213a1.
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: IG3, monoclonal
• Espèces réactives: mouse
• Conditionnement: antibody small pack of 25 μg
• Technique(s): flow cytometry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_598751
• Numéro d'accès UniProt: O09030
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Il13Ra1(16164)

Description

Description générale

Interleukin-13 receptor subunit alpha-1 (UniProt: O09030; also known as IL-13 receptor subunit alpha-1, IL-13R subunit alpha-1, IL-13R-alpha-1, IL-13RA1, Interleukin-13-binding protein, Novel cytokine receptor 4, NR4, CD213a1) is encoded by the Il13ra1 (also known as Il13r, Il13ra) gene (Gene ID: 16164) in murine species. Interleukin 13 (IL-13) is a T cell derived cytokine involved in the regulation of inflammatory and immune responses. IL-13RA1 is a single-pass type I membrane protein that binds with low affinity to interleukin-13 (IL13). Together with IL-4RA it forms a functional receptor for IL13. It is also reported to serve as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL-2) binding activity. IL13-RA1 is expressed I Spleen, liver, thymus, heart, lung, kidney, testis, stomach, brain, skin, and colon. However, is not expressed in the skeletal muscle. It is also shown to bind tyrosine kinase TYK2, and thereby mediate the signaling processes that lead to the activation of JAK1, STAT3, and STAT6 induced by IL-13 and IL-4. IL-13RA1 is synthesized with a signal peptide (aa 1-25) that is cleaved off to produce mature form. It contains an extracellular domain (aa 26-340), a helical domain (aa 341-364), and a cytoplasmic region (aa 365-424). It also contain two fibronectin type III domains (aa 32-121 and 224-336). The WSXWS motif (aa 324-328) appears to be essential for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

Spécificité

Clone IG3 detects Interleukin-13 receptor subunit alpha-1 in murine cells.

Immunogène

Recombinant mouse Interleukin-13 receptor subunit alpha-1.

Application

Anti-IL13R alpha 1, clone IG3, Cat. No. MABF2060, is a mouse monoclonal antibody that detects Interleukin-13 receptor subunit alpha-1 and has been tested for use in Flow Cytometry, Immunoprecipitation, and Western Blotting.

Flow Cytometry Analysis: A representative lot detected IL13R alpha 1 in Flow Cytometry applications (Haymaker, C.L., et. al. (2012). J Immunol. 188(7):3208-16; Dhakal, M., et. al. (2014). Eur J Immunol. 44(3):842-55).

Western Blotting Analysis: A representative lot detected IL13R alpha 1 in Western Blotting applications (Haymaker, C.L., et. al. (2012). J Immunol. 188(7):3208-16; Dhakal, M., et. al. (2014). Eur J Immunol. 44(3):842-55).

Immunoprecipitation Analysis: A representative lot detected IL13R alpha 1 in Immunoprecipitation applications (Dhakal, M., et. al. (2014). Eur J Immunol. 44(3):842-55).

Research Category
Inflammation & Immunology

Qualité

Evaluated by Western Blotting in mouse spleen tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected IL13R alpha 1 in 10 µg of mouse spleen tissue lysate.

Description de la cible

~53 kDa observed; 48.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash