MABE952

Anti-Histone H3.1 Antibody, clone 1D4F2

clone 1D4F2, 1 mg/mL, from rat

• Synonyme(s) : Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rat
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1D4F2, monoclonal
• Espèces réactives: human, mouse
• Concentration: 1 mg/mL
• Technique(s): ChIP: suitable (ChIP-seq); immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Numéro d'accès NCBI: NP_003520
• Numéro d'accès UniProt: P68431
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... HIST1H3F(8968)

Description

Description générale

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogène

KLH-conjugated linear peptide corresponding to human Histone H3.1.

Application

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Histone H3.1 in HeLa cells.

Immunocytochemistry Analysis: A 1:2,000 dilution from a representative lot detected Histone H3.1 in NIH/3T3 cells (Prof. Taro Tachibana, Cell Engineering Corporation.).

Alexa Fluor^™ is a registered trademark of Life Technologies.

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected Histone H3.1 in C2C12 cells (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3.1 (Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.).

Chromatin Immunoprecipitation Sequencing Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3.1 (Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.).

This Anti-Histone H3.1 Antibody, clone 1D4F2 is validated for use in western blotting, ICC, IP, ChIP & ChIP-seq for the detection of Histone H3.1.

Qualité

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 1 µg/mL of this antibody detected Histone H3.1 in 10 µg of HeLa acid extract.

Description de la cible

~17 kDa observed

Forme physique

Format: Purified

Informations légales

ALEXA FLUOR is a trademark of Life Technologies

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable