MABE330

Anti-c-ErbB2/c-Neu Antibody, clone 3B5

clone 3B5, from mouse

• Synonyme(s) : Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, CD340
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 3B5, monoclonal
• Espèces réactives: human
• Technique(s): immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_001005862
• Numéro d'accès UniProt: P04626
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ERBB2(2064)

Description

Description générale

The ErbB2 (HER2, c-Neu, ErbB2, c-erbB2, or HER2/neu) protein is member of the human epidermal growth factor receptor (HER) family. It is a receptor tyrosine kinase containing an intracellular kinase domain and an extracellular ligand domain, although no endogenous ligand has yet been found. ErbB2 dimerizes with ErbB1, 3, and 4, and can activate different pathways including the PI3K, PLCγ, and MAPK pathways, depending on the ErbB receptor involved. Coupling with ErbB3 activates the PI3K pathway, whereas coupling with any of these three receptors can induce the MAPK pathway. ErbB2 signaling promotes cell proliferation and inhibits apoptosis. ErbB2 is overexpressed in various cancers and may play a role in the development and metastasis of gliomas, and ovarian, breast, lung, and gastric cancer.

Immunogène

Linear peptide corresponding human c-ErbB2/c-Neu.

Application

Anti-c-ErbB2/c-Neu Antibody, clone 3B5 is a Mouse Monoclonal Antibody for detection of c-ErbB2/c-Neu also known as MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2 or CD340, has been validated in WB & IHC.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected c-ErbB2/c-Neu in human breast cancer tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Qualité

Evaluated by Western Blot in SK-BR-3 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected c-ErbB2/c-Neu in 10 µg of SK-BR-3 cell lysate.

Description de la cible

~185 kDa observed. Uniprot describes four isoforms at ~138 kDa (isoform 1), ~63 kDa (isoform 2), ~71 kDa (isoform 3), and ~137 kDa (isoform 4), which can be highly glycosylated and phosphorylated. This antibody may detect one or more isoforms. Uncharacterized bands may be observed at ~27 kDa and ~38 kDa in some cell lysates.

Liaison

Replaces: 04-1127

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
SK-BR-3 cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable