MABC609

Anti-Thymidylate Kinase/TMPK Antibody, clone 1A5.1

clone 1A5.1, from mouse

• Synonyme(s) : Thymidylate kinase, Thymidylate Kinase/TMPK, dTMP kinase
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1A5.1, monoclonal
• Espèces réactives: human
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_001158503
• Numéro d'accès UniProt: P23919
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TK1(7083)

Description

Description générale

Thymidylate kinase (EC 2.7.4.9; UniProt P23919; also known as dTMP kinase) is encoded by the DTYMK (also known as CDC8, PP3731, TMPK, TYMK) gene (Gene ID 1841) in human. Thymidylate kinase (TMPK) is an ubiquitously expressed enzyme of the dTTP synthesis pathway involved in DNA synthesis. TMPK catalyzes the phosphorylation of thymidine 5′-monophosphate (dTMP) in the presence of ATP and Mg(2+) to form thymidine 5′-diphosphate (dTDP), which is then further phosphorylated by nucleoside-diphosphate kinase (NDK) to thymidine 5′-triphosphate (dTTP). Aside from its normal physiological role, TMPK activity is essential for the activation of nucleoside analogs-based prodrugs, such as the clinically used anti-HIV prodrugs AZT and d4T, which need to be converted to the triphosphate forms for their pharmacological activities. Inhibition of TMPK activity is reported to augment the potency of anticancer agent doxorubicin against human colon cancer cells regardless of their p53 status. In addition, lung cancers with LKB1-mutations are reported to show deficits in nucleotide metabolism and exhibit hypersensitivity to DTYMK inhibition.

Immunogène

GST-tagged recombinant protein corresponding to human Thymidylate Kinase/TMPK.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-TMPK Antibody, clone 1A5.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Thymidylate Kinase .

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Thymidylate Kinase/TMPK in 10 µg of MCF-7 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Thymidylate Kinase/TMPK in human breast cancer and human ovarian cancer tissue.

Qualité

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Thymidylate Kinase/TMPK in 10 µg of Jurkat cell lysate.

Description de la cible

~24 kDa observed

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable