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Key Documents

MAB8051

Sigma-Aldrich

Anti-Adenovirus Antibody, clone 2/6

clone 2/6, Chemicon®, from mouse

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified immunoglobulin

Clone

2/6, monoclonal

Espèces réactives

human

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable

Isotype

IgG1κ

Conditions d'expédition

wet ice

Description générale

Adenoviruses are 65-80 nm, non-enveloped, regular icosahedron pathogens often associated with respiratory and gastrointestinal illness as well as conjunctivitis. Well over 40 types of adenoviruses have currently been recognized. In addition to their deleterious effects, adenoviruses have been used in vaccine production and in gene therapy. They also have the ability to trigger cell proliferation by interfering with host cellular anti-oncogenes. Since they are easy to prepare, multiple quickly and efficiently, and have the ability to infect and deliver their genome (including researcher-created constructs) to the nucleus of a variety of post-mitotic cells, adenoviruses are extremely valuable in research and medical applications ranging from virus/host cell interaction to the insertion of novel genes or regulatory mechanisms into eukaryotic cellular systems, to delivery of therapeutic anti-cancer genes.

Spécificité

All 41 serotypes of Adenovirus.

Application

Detect Adenovirus using this Anti-Adenovirus Antibody, clone 2/6 validated for use in ELISA, FC, IF, IH.
ELISA

IFA: acetone fixed cell preparations or tissues.

FACS: {McNees, 2004}

Dilute with buffer pH 7.5-8.0 to desired working volume.

For extensive dilution, protein containing or other stabilizing medium should be used.

Final working dilutions must be determined by end user.

Forme physique

Format: Purified
Protein A-purified immunoglobulin. Liquid at 1 mg/mL in 20 mM phosphate buffer, containing 25 mM NaCl and 0.1% sodium azide, pH 7.6

Stockage et stabilité

Maintain at 2-8°C in convenient aliquots for up to 12 months. Do not store in a diluted format.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

An outbreak of epidemic keratoconjunctivitis in a regional ophthalmology clinic in New South Wales.
K A Viney,P J Kehoe,B Doyle,V Sheppeard,A R Roberts-Witteveen,H Semirli,K A McPhie et al.
Epidemiology and Infection null
Postinternalization inhibition of adenovirus gene expression and infectious virus production in human T-cell lines.
McNees, AL; Mahr, JA; Ornelles, D; Gooding, LR
Journal of virology null
Protein crystals in Adenovirus type 5-infected cells: requirements for intranuclear crystallogenesis, structural and functional analysis.
Franqueville, L; Henning, P; Magnusson, M; Vigne, E; Schoehn, G; Blair-Zajdel, ME; Habib et al.
Testing null
Modeling adenovirus latency in human lymphocyte cell lines.
Zhang, Y; Huang, W; Ornelles, DA; Gooding, LR
Journal of virology null

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