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Key Documents

MAB4037

Sigma-Aldrich

Anti-PTEN Antibody, CT, clone A2b1

clone A2b1, Chemicon®, from mouse

Synonyme(s) :

MMAC1, TEP1

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

A2b1, monoclonal

Espèces réactives

human, rat, mouse

Fabricant/nom de marque

Chemicon®

Technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG1

Adéquation

not suitable for immunohistochemistry (Paraffin)

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

Spécificité

Specifically recognizes PTEN*, a tumor suppressor protein located on human chromosome 10 (Steck, ′97; Li, ′97; Rhei, ′97) that is often mutated in various types of advanced cancers (Rhei, ′97). The PTEN protein exhibits protein phosphatase activity (Myers, ′97) and can suppress the growth of glioma cells (Furnari, ′97). The antibody recognizes human and mouse PTEN and is specific for the C-terminus. Other species not tested. Reactivity with human fibroblasts, breast epithelial and lung cancer cell lines has been confirmed by western blot, and antibody specificity has been confirmed by peptide blocking studies.

*PTEN is covered under U.S. patents 6,262,242, 6,482,795 and U.S. patent application 10/299,003.

Immunogène

Anti-PTEN was generated by immunization with a 10 amino acid peptide corresponding to amino acids 387-400 near the C-terminus of human PTEN.
Epitope: C-terminus

Application

Anti-PTEN Antibody, C-terminus, clone A2b1 is an antibody against PTEN for use in IC, IH, IP & WB.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
Western blot 1:100 (55 kDa)

Immunocytochemistry

Immunohistochemistry: 1:20 to 1:50 for acetone-fixed, fresh frozen tissue. Not effective for paraffin sections.

Immunoprecipitation 1:10 to 1:20

Protocol for immunohistochemistry of frozen tissue sections on glass slides.

1. Fix sections for 5 minutes with cold acetone.

2. Air dry

3. Wash sections with PBS

4. Add blocking solution (horse serum) for 20 minutes

5. Add diluted PTEN antibody and incubate for 1 hour at room temperature.

6. Wash with PBS

7. Add biotinylated secondary antibody (horse anti-mouse, or equivalent).

8. Incubate for 30 minutes at room temperature.

9. Wash with PBS

10. Detect with ABC (Avidin-Peroxidase-Complex) or equivalent.

11. Wash with PBS

12. Develop with DAB substrate.

13. Hematoxylin dye may be used as a counterstain for visualizing nuclei.Breast tumor tissue gives good staining. Prostate tissue does not stain.Optimal working dilutions must be determined by end user.

Description de la cible

55 kDa

Liaison

Replaces: 04-409

Forme physique

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified

Stockage et stabilité

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Remarque sur l'analyse

Control
Breast tumor tissue

Purified active kinase is Catalogue Number 14-488

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Carsten B Schmidt-Weber et al.
European journal of immunology, 32(4), 1196-1204 (2002-04-05)
The phosphatidylinositol phosphatase gene PTEN is a dual specific phosphatase acting on phospho amino acids but also on three phosphorylated inositol phospholipids. Present results demonstrate that PTEN is inducible by costimulatory signals in human CD4(+) T cells. PTEN expression was
Delira Robbins et al.
FEBS letters, 586(23), 4108-4113 (2012-11-06)
Our recent studies suggest a unique role of p53 during the early stage of cancer development. However, how p53 activation is regulated during TPA treatment remains elusive. We used murine skin epidermal JB6 promotion-sensitive (P+) and promotion resistant (P-) cells
Joseph O Humtsoe et al.
Molecular and cellular biology, 30(7), 1593-1606 (2010-02-04)
Endothelial cell (EC) migration, cell-cell adhesion, and the formation of branching point structures are considered hallmarks of angiogenesis; however, the underlying mechanisms of these processes are not well understood. Lipid phosphate phosphatase 3 (LPP3) is a recently described p120-catenin-associated integrin
Janice B B Lam et al.
PloS one, 4(3), e4968-e4968 (2009-03-26)
Adiponectin is an adipokine possessing beneficial effects on obesity-related medical complications. A negative association of adiponectin levels with breast cancer development has been demonstrated. However, the precise role of adiponectin deficiency in mammary carcinogenesis remains elusive. In the present study
Danyu Cui et al.
Molecular medicine reports, 16(5), 7813-7820 (2017-09-26)
A number of short noncoding microRNAs (miRs) have been demonstrated to be highly expressed in many kidney diseases such as renal cancer and lupus nephritis (LN); however, these results have not been extensively investigated. The aim of the present study

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