MAB3776

Anti-MDM2 Antibody, clone SMP14

clone SMP14, Chemicon^®, from mouse

• Synonyme(s) : Murine Double Minute 2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: SMP14, monoclonal
• Application: IHC (p), IP
• Citations: 4

Propriétés

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: SMP14, monoclonal
• species reactivity: rat, human, mouse
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable
• isotype: IgG1
• NCBI accession no.: NM_002392.2, NM_006878.2, NM_006879.2, NM_006881.2, NM_006882.2
• UniProt accession no.: Q00987
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... MDM2(4193) ; mouse ... Mdm2(17246) ; rat ... Mdm2(314856)

Description

General description

MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated (Zhang & Prives, 2001). SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.

Immunogen

Synthetic peptide corresponding to residues 154-167 (CSRPSTSSRRRAISE) of human MDM2 cross-linked to KLH using glutaldehyde.

Application

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

RNA Binding Protein (RBP)

Use Anti-MDM2 Antibody, clone SMP14 (Mouse Monoclonal Antibody) validated in IP, IHC, IHC(P) to detect MDM2 also known as Murine Double Minute 2.

Western blot

Immunohistochemistry (Frozen and Paraffin-embedded sections)

Immunoprecipitation

ELISA

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Recognizes human MDM2.

Physical form

Format: Purified

Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide

Analysis Note

Control
POSITIVE CONTROL: Breast carcinoma

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Classe de stockage: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable