MAB342-AF647

Anti-Galactocerebroside Antibody, clone mGalC Antibody, Alexa Fluor^™ 647 conjugate

clone mGalC, from mouse, ALEXA FLUOR^™ 647

• Synonyme(s) : Galactocerebrocide, Galactosylceramide, GalC, GalCer
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conjugué: ALEXA FLUOR^™ 647
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: mGalC, monoclonal
• Espèces réactives: rabbit, bovine, human, rat, mouse
• Technique(s): immunocytochemistry: suitable
• Isotype: IgG3
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... GALC(2581)

Description

Description générale

Galactocerebroside (GalCer or GalC) is the most frequently found glycosphingolipid in animals. GalCer consist of a ceramide linked to a single galactose residue at the 1-hydroxyl moiety. Cerebrosides are concentrated in nervous tissues where they increase from about 0.02% of the dry weight in human fetal brain to 2% in gray matter and 12% in white matter of adult brain. GalCer is a specific cell-surface antigenic marker for oligodendrocytes and schwann cells. GalCer is the largest single component of the myelin sheath of nerves. GalCer synthesis therefore serves as a measurement of myelin formation or remyelination. Krabbe disease is an autosomal recessive sphingolipidosis caused by deficient activity of the lysosomal hydrolase galactosylceramide beta-galactosidase (GALC). GALC mediates the degradation of GalCer and other terminal beta-Gal-containing sphingolipids, including psychosine (galactosylsphingosine). Increased psychosine levels are believed to lead to widespread destruction of oligodendroglia in the CNS and to subsequent demyelination.

Spécificité

Galactocerebroside (GalC), sulfatide, psychosine and other galactolipids. Cross-reacts with the sulfatide ester of GalC, but to a 16-fold lesser extent. No cross-reactivity with sphingosine, ceramide, mixed ganglioside or glucocerebroside. Binds specifically to the surface of oligodendrocytes and Schwann cells (Ranscht, B. et al. (1982) Proc. Natl. Acad. Sci. U. S. A. 79(8):2709-2713).

Target lipid structure is not species-specific.

Immunogène

Synaptic plasma membranes from bovine hippocampus (Ranscht, B. et al. (1982) Proc. Natl. Acad. Sci. U. S. A. 79(8):2709-2713).

Application

Anti-Galactocerebroside, clone mGalC, Alexa Fluor^™ 647 conjugate, Cat. No. MAB342-AF647, is a highly specific mouse monoclonal antibody conjugate that targets galactocerebroside (GalC) and has been tested in Immunocytochemistry.

Research Category
Neuroscience

The unconjugated antibody (Cat. No. MAB342) and other conjugates (Cat. Nos. FCMAB312F, MAB342-AF555 and MAB342A4) are also available for ELISA, Flow Cytometry, Immunocytochemistry, and Immunofluorescence applications.

Qualité

Evaluated by Immunocytochemistry in rat Schwann cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody immunostained non-permeabilzed rat schwann cells.

Forme physique

Protein A purified.

Purified mouse IgG3 Alexa Fluor^™ 647 conjugate in PBS with 1.5% BSA and 0.09% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations légales

ALEXA FLUOR is a trademark of Life Technologies

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable