GF092

TRAIL Protein, Recombinant human

Human TRAIL (TNF-Related Apoptosis Inducing Ligand), also called APO2 Ligand, is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells.

• Synonyme(s) : Apo2 Ligand
• Code UNSPSC : 12352202
• eCl@ss : 32160405
• Nomenclature NACRES : NA.75

Propriétés

• Source biologique: human
• Niveau de qualité: 100
• Produit recombinant: expressed in E. coli
• Pureté: >98% (SDS-PAGE and HPLC)
• Forme: solid
• Poids mol.: 19.6 kDa
• Fabricant/nom de marque: Chemicon^®
• Technique(s): cell culture | mammalian: suitable
• Impuretés: <0.1 ng/μg Endotoxin (of TRAIL/Apo2L; 1EU/μg)
• Entrée: sample type hematopoietic stem cell(s)
• Adéquation: suitable for molecular biology
• Numéro d'accès NCBI: NM_003810.2
• Numéro d'accès UniProt: P50591
• Conditions d'expédition: dry ice
• Informations sur le gène: human ... TNFSF10(8743)

Description

Description générale

Research area: CancerTNF-Related Apoptosis-Inducing Ligand (TRAIL) is a cytotoxic and type II membrane protein. It belongs to the tumor necrosis factor (TNF) superfamily. TRAIL is expressed by the cells of the immune system including natural killer cells, macrophages, T cells, and dendritic cells. TRAIL Protein, Recombinant human is a 19.6 kDa protein

Application

TRAIL Protein, Recombinant human has been used as anapoptosis inducer: to extract cell-free DNA (cfDNA) from various cell linesalong with BV6 to study its anticancer effects in breast cancer cell lines, tostudy its effects of ethanol-induced interferon expression on TRAIL release in neurons

Actions biochimiques/physiologiques

TNF-Related Apoptosis-Inducing Ligand (TRAIL) promotes rapid apoptosis in tumor cells while sparing normal cells. Thisproperty of TRAIL makes it an important biotherapeutic agent in cancer therapy.It induces apoptosis by binding to two death signaling receptors, DR4 and DR5expressed on cell membranes.

Forme physique

Sterile filtered, then lyophilized from 0.3 mg/mL in 10mM Sodium Phosphate + 110 mM NaCl.

Stockage et stabilité

The lyophilized protein may be stored at room temperature for a few weeks, but is best stored at -20°C. After a quick spin, reconstitute in sterile water to a concentration of 0.5-1.0 mg/mL. This solution can be diluted into other buffered solutions and stored at 4ºC for 1 week or -20ºC for future use. Reconstituted GF092 should be stored in undiluted aliquots at -20°C for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3