CP70
Anti-Fibronectin (Ab-3) Mouse mAb (FBN11)
liquid, clone FBN11, Calbiochem®
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A propos de cet article
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
FBN11, monoclonal
Species reactivity:
rat, human, mouse
Application:
—
Citations:
7
Service technique
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Laissez-nous vous aiderbiological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
FBN11, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, human, mouse
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Frozen Sections (0.5-1 µg/mL)
Immunoblotting (0.5-1 µg/mL)
Immunofluorescence
Immunoprecipitation (2 µg/mg protein lysate)
Paraffin Sections (0.5-1 µg/mL, heat pre-treatment required))
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... FN1(2335)
mouse ... Fn1(14268)
rat ... Fn1(25661)
General description
Anti-Fibronectin (Ab-3), mouse monoclonal, clone FBN11, recognizes dimeric fibronectin in SWI56 cells and normal kidney. It is validated for use in WB, IF, IP & IHC (frozen and paraffin sections).
Protein G purified mouse monoclonal antibody. Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin.
Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin in SWI56 cells and normal kidney tissue.
Immunogen
recombinant human fibronectin
Application
Frozen Sections (0.5-1 µg/ml)
Immunoblotting (0.5-1 µg/ml)
Immunofluorescence (see comments)
Immunoprecipitation (2 µg/mg protein lysate)
Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required)
Immunoblotting (0.5-1 µg/ml)
Immunofluorescence (see comments)
Immunoprecipitation (2 µg/mg protein lysate)
Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required)
Packaging
Please refer to vial label for lot-specific concentration.
Analysis Note
Positive Control
SW 156 cells or normal kidney tissue
SW 156 cells or normal kidney tissue
Other Notes
Clark, R.A. 2001. Ann. N.Y. Acad. Sci.936, 355.
Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta1470, O9.
Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum.29, 252.
Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47.
Rouslahti, E. and Pierschbacher, M.D. 1986. Cell44, 517.
Pytela, R., et al. 1985. Cell40, 191.
Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta1470, O9.
Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum.29, 252.
Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47.
Rouslahti, E. and Pierschbacher, M.D. 1986. Cell44, 517.
Pytela, R., et al. 1985. Cell40, 191.
Exhibits staining of the connective tissue matrix when used in immunohistochemistry. This antibody is suitable for both reduced and non-reduced protein by immunoblotting. For staining of formalin-fixed tissues, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. This antibody has also been reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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