CBL407

Anti-PCNA Antibody, clone PC10

clone PC10, Chemicon^®, from mouse

• Synonyme(s) : Proliferating Cell Nuclear Antigen, DNA Polymerase delta Processivity Factor
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: PC10, monoclonal
• Application: ELISA, FACS, IHC, IP, WB
• Citations: 24

Propriétés

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: PC10, monoclonal
• species reactivity: vertebrates, invertebrates
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, flow cytometry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG2a
• NCBI accession no.: NM_022381.3
• UniProt accession no.: P04961
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PCNA(5111)

Description

General description

Proliferating cell nuclear antigen (UniProt: P04961; also known as PCNA) is encoded by the Pcna gene (Gene ID: 25737) chez le rat. ′ ′′′ It is reported to colocalize with CREBBP, EP300 and POLD1 to sites of DNA damage. It acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote post-replication repair. PCNA can undergo phosphorylation on tyrosine 211 by EGFR that stabilizes chromatin-associated PCNA. Following DNA damage, it can undergo monoubiquitination to stimulate direct bypass of DNA lesions by specialized DNA polymerases or polyubiquitination to promote recombination-dependent DNA synthesis across DNA lesions by template switching mechanisms. Mutations in PCN gene are known to cause Ataxia-telangiectasia-like disorder 2 (ATLD2) that is characterized by developmental delay, ataxia, hearing loss, and short stature.

Immunogen

Rat PCNA induced in the Protein A expression vector pR1T2T.

Application

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Domaine de recherche
Épigénétique et fonction nucléaire

Sous-domaine de recherche
Cycle cellulaire, réplication et réparation de l'ADN

Biochem/physiol Actions

Clone PC10 recognizes PCNA from all vertebrate and insect species tested and has been used to identify transformed cells (Kurki, 1988), proliferating cells in solid tumors (Smetana, 1983), and blast cells in leukemia patients (Takasaki, 1984). Immunohistochemistry with clone PC10 has been used to study the expression of PCNA in paraffin sections of normal tissues and lymphoid neoplasms (Hall, 1990). In proliferating cells, PCNA staining pattern is predominantly nuclear. By western blot, the antibody detects a polypeptide migrating at 36 kDa with an isoelectric point of 4.8.

Physical form

μ Il est recommandé que chaque laboratoire détermine le titre de travail optimal à utiliser dans sa propre application.

Format : Produit purifié

Preparation Note

À conserver à +4 °C pour une utilisation dans le mois suivant l'achat ; pour un stockage à long terme, diviser l'anticorps en aliquotes et les conserver à -20 °C. Avoid repeated freeze/ thawing.

Analysis Note

Témoin
TÉMOIN POSITIF : Tonsil or reactive lymph node.

Other Notes

Concentration : pour connaître la concentration spécifique du lot, voir le certificat d'analyse.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Sauf indication contraire dans notre catalogue ou toute autre documentation associée au(x) produit(s), nos produits sont uniquement destinés à la recherche et ne sauraient être utilisés à d'autres fins, ce qui inclut, sans s'y limiter, les utilisations commerciales non autorisées, les utilisations diagnostiques in vitro, les utilisations thérapeutiques ex vivo ou in vivo, ou tout type de consommation ou d'application chez l'être humain ou chez l'animal.

Informations sur la sécurité

• Classe de stockage: 12 - Non Combustible Liquids
• wgk: WGK 2