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Key Documents

CBL188

Sigma-Aldrich

Anti-Clathrin Antibody, clone CHC5.9

clone CHC5.9, Chemicon®, from mouse

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

CHC5.9, monoclonal

Espèces réactives

bovine, amphibian, rat, pig, human

Fabricant/nom de marque

Chemicon®

Technique(s)

immunohistochemistry: suitable
western blot: suitable

Isotype

IgM

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CLTC(1213)

Spécificité

This antibody reacts with the clathrin heavy chain from a diverse range of species. In nervous tissue it cross-reacts with another coated vesicle polypeptide of 190 kDa which is not present in isolated clathrin triskelions. In some epithelial cells of certain species the antibody may cross-react with a cytokeratin component. It detects coated vesicles from different organs and tissues (e.g. brain, ovary, mammary gland). : Recognizes bovine, rat and amphibian

Immunogène

Coated vesicles (clathrin) of bovine brain.

Application

Anti-Clathrin Antibody, clone CHC5.9 is an antibody against Clathrin for use in WB, IH.
Marker for detection of receptor mediated endocytosis

Suitable for frozen sections

Western blotting KNOW SPECIES

Optimal working dilutions must be determined by the end user.
Research Category
Epitope Tags & General Use
Research Sub Category
Organelle & Cell Markers

Forme physique

Format: Purified
The antibody was purified by gel filtration and is presented as 100μg of lyophilysed material. Reconstitute with 1ml distilled water. Final buffer composition is PBS, pH 7.4.

Stockage et stabilité

Maintain at 2-8°C for up to 12 months from date of receipt.

Remarque sur l'analyse

Control
Positive reactions using HeLa, SV-40 and RVF-SMC cultured cell lines.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Yvonne G Y Chan et al.
Cellular microbiology, 11(4), 629-644 (2009-01-13)
Rickettsia conorii, an obligate intracellular tick-borne pathogen and the causative agent of Mediterranean spotted fever, binds to and invades non-phagocytic mammalian cells. Previous work identified Ku70 as a mammalian receptor involved in the invasion process and identified the rickettsial autotransporter
Hisako Furukawa et al.
Laboratory investigation; a journal of technical methods and pathology, 84(7), 828-835 (2004-05-04)
Prion protein (PrP) is known to accumulate in some non-neuronal tissues under conditions unrelated to prion diseases. The biochemical and biological nature of such accumulated PrP molecules, however, has not been fully evaluated. In this study, we established experimental myopathy
Eileen Dahlke et al.
Cells, 12(1) (2023-01-09)
The neonatal Fc receptor (FcRn) is highly expressed in the renal proximal tubule and is important for the reclamation of albumin by cellular transcytosis to prevent its loss in the urine. The initial event of this transcellular transport mechanism is

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