ABT42

Anti-Myosin-VI Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Myosin-VI, Unconventional myosin-6
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: canine, human, mouse
• Réactivité de l'espèce (prédite par homologie): rat (based on 100% sequence homology)
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_004990
• Numéro d'accès UniProt: Q9UM54
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... MYO6(4646)

Description

Description générale

Myosin-VI has been found in the membrane ruffles at the leading edge of the Golgi complex. Myosin-VI moves along the actin in the opposite way of other known myosins to date, toward the minus end. It can exist as a dimer and a monomer and is thought to function as a non-processive motor with a 18nm working stroke. Myosin-VI has also been found in the brain, specifically the synapses. Myosin-VI deficiency (Snell’s waltzer disease) in the hippocampus causes a smaller number of synapses, short dendritic spines and astrogliosis. Myosin-VI may also be important in clathrin-mediated endocytosis of acid-type glutamate receptors. Recent research has implicated myosin-VI in the enrichment of proteins on the surface of axons.

Immunogène

KLH-conjugated linear peptide corresponding to human Myosin-VI.

Application

Anti-Myosin-VI Antibody detects level of Myosin-VI & has been published & validated for use in WB & IC.

Immunocytochemistry analysis: A 1:500 dilution from a representative lot detected Myosin-VI in NIH/3T3 and A431 cells.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Qualité

Evaluated by Western Blot in MDCK cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Myosin-VI in 10 µg of MDCK cell lysate.

Description de la cible

~150 kDa observed.
An uncharacterized band appears at ~55 kDa in some lysates.

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
MDCK cell lysate

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable