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Key Documents

ABS1051

Sigma-Aldrich

Anti-Mitoferrin-1/Mfrn1 Antibody

serum, from rabbit

Synonyme(s) :

Mitoferrin-1, Mitoferrin-1/Mfrn1, Mitochondrial iron transporter 1, Mitochondrial solute carrier protein, Solute carrier family 25 member 37

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

serum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Espèces réactives

mouse

Technique(s)

western blot: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

Description générale

Mitoferrin-1 (UniProt Q920G8; also known as mitochondrial iron transporter 1, mitochondrial solute carrier protein, solute carrier family 25 member 37) is encoded by the Slc25a37 (also known as Mfrn, Mfrn1, Mscp) gene (Gene ID 67712) in murine species. Mitoferrin-1 and mitoferrin-2 (Slc25a28, Mfrn2) mediate iron import into mitochondria. Mitoferrin-2 is expressed ubiquitously, whereas mitoferrin-1 is specifically induced in differentiating erythroid cells. Mfrn1 knockout in zebrafish and mice disrupts heme and [Fe-S] cluster synthesis as a result of a severe reduction in mitochondrial iron in erythroid progenitors.

Spécificité

This antiserum does not react with spliced isofrms 2, 3, 4, and 5.

Immunogène

Epitope: C-terminus
Linear peptide corresponding to the C-terminus of mouse Mitoferrin-1/Mfrn1.

Application

Research Category
Signaling
Research Sub Category
Developmental Signaling
This Anti-Mitoferrin-1/Mfrn1 Antibody is validated for use in Western Blotting for the detection of Mitoferrin-1/Mfrn1.
Western Blotting Analysis: A 1:2,000 dilution of this antiserum detected mitoferrin-1 upregulation in mitochondria lysates from mouse erythroleukemia (MEL) cultures chemically induced to undergo erythroid maturation (Courtesy of Dr Barry H. Paw, Brigham and Women′s Hospital, Boston, MA).
Western Blotting Analysis: A representative lot detected reduced mitoferrin-1 expression due to heterozygous gene trap insertion in the Mfrn1 gene (Mfrn1+/gt) and loss of mitoferrin-1 expression due to homozygous Mfrn1 gene insertion (Mfrn1gt/gt) in mouse embryonic stem cells (mESCs) undergoing primitive erythropoiesis (Chung, J., et al.(2014). J Biol Chem. 289(11):7835-7843).
Western Blotting Analysis: A representative lot detected upregulated mitoferrin-1 expression following DMSO-induced erythroid maturation in mouse erythroleukemia (MEL) cultures (Shah, D.I., et al. (2012). Nature. 491(7425):608-612).

Qualité

Evaluated by Western Blotting in mouse embryonic stem (mES) cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antiserum detected mitoferrin-1 in mouse embryonic stem (mES) cell lysates.

Description de la cible

~34 kDa observed. Uncharacterized band(s) may appear in some lysates.

Forme physique

Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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