ABE558

Anti-Ku70 Protein Antibody

from rabbit

• Synonyme(s) : X-ray repair cross-complementing protein 6, 5′-deoxyribose-5-phosphate lyase Ku70, 5′-dRP lyase Ku70, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box-binding factor 75 kDa subuni
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human
• Technique(s): immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• Numéro d'accès NCBI: NP_001460
• Numéro d'accès UniProt: P12956
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... XRCC6(2547)

Description

Description générale

Lupus Ku autoantigen protein p70 (Ku70) is also known as X-ray repair cross-complementing protein 6 (XRCC6), 5′-dRP lyase Ku70, CTC box-binding factor 75 kDa subunit (CTC75, CTCBF) or Thyroid-lupus autoantigen (TLAA). Ku70 is a single stranded DNA-dependent ATP-dependent helicase that plays a role in chromosome translocation and DNA non-homologous end joining (NHEJ) required for double-strand break repair and recombination. The XRCC5/6 (Ku80/Ku70) dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK, and that dimer, together with APEX1, acts as a negative regulator of transcription. Individuals with systemic lupus erythematosus (SLE) and related disorders produce extremely large amounts of autoantibodies to XRCC5 (Ku80) and XRCC6 (Ku70).

Spécificité

This antibody recognizes Ku70 Protein near N-Terminus.

Immunogène

Epitope: Near N-Terminus

KLH-conjugated linear peptide corresponding to human Ku70 Protein near N-Terminus.

Application

Detect Ku70 Protein using this rabbit polyclonal antibody, Anti-Ku70 Protein Antibody validated for use in western blotting, IP & IHC (Paraffin).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Ku70 Protein in 0.5 mg of HeLa cell lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Ku70 Protein in human prostate carcinoma cells and human testis tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Qualité

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ku70 Protein in 10 µg of HeLa cell lysate.

Description de la cible

~70 kDa observed

Liaison

Replaces: AB1358

Forme physique

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
HeLa cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable