ABC439

Anti-PKM1 Antibody

serum, from rabbit

• Synonyme(s) : Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tumor M2-PK, p58
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: serum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human, mouse
• Technique(s): immunohistochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_001193725
• Numéro d'accès UniProt: P14618
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PKM(5315)

Description

Description générale

Pyruvate kinase isozymes M1/M2 (PKM2) is also called Cytosolic thyroid hormone-binding protein (CTHBP), Opa-interacting protein 3 (OIP-3), Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1 (THBP1) and p58. PKM2 is a glycolytic enzyme that assists in generating ATP. PKM2 also stimulates POU5F1-mediated transcriptional activation, and plays a role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. This control of glycolysis and is important for tumor cell proliferation and survival. PKM2 is specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, and cancer cells.

Spécificité

This anitbody is specific to PKM1.

Immunogène

Linear peptide corresponding to human PKM1.

Application

Anti-PKM1 Antibody detects level of PKM1 & has been published & validated for use in PKM1.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PKM1 in 10 µg of mouse brain and RAW264.7 tissue lysate.
Western Blotting Analysis: A representative lot detected PKM1 in HEK293 cell lysate (Christofk, R. H., et al. (2008). Nature. 452:230-233).
Immunohistochemistry Analysis: A representative lot detected PKM1 in HEK293 tissue (Christofk, R. H., et al. (2008). Nature. 452:230-233).

Qualité

Evaluated by Western Blotting in C2C12 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PKM1 in 10 µg of C2C12 cell lysate.

Description de la cible

~58 kDa observed

Forme physique

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable