Diluting the Poly-L-Ornithine solution in PBS to achieve the desired concentration is not recommended; instead, use sterile water as instructed. Please follow the provided guidelines carefully for optimal results. For more information, refer to: https://www.sigmaaldrich.com/en/product/mm/a004m
A-004-M
Poly-ʟ-Ornithine
synthetic, liquid, 0.1 mg/mL, suitable for cell culture
Synonyme(s) :
Poly-L-Ornithine Solution (0.01%), A-004-M, Sigma-Aldrich
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A propos de cet article
UNSPSC Code:
12352209
eCl@ss:
32160801
NACRES:
NA.75
Biological source:
synthetic
Form:
liquid
Technique(s):
cell attachment: suitable, cell culture | mammalian: suitable
Concentration:
0.01 % (w/v), 0.1 mg/mL
Mol wt:
Mw 78000 Da
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Laissez-nous vous aiderNom du produit
Poly-L-Ornithine Solution (0.01%),
biological source
synthetic
Quality Segment
needle size
2.0 mm
form
liquid
mol wt
Mw 78000 Da
manufacturer/tradename
NovaSeptum®
concentration
0.01 % (w/v), 0.1 mg/mL
technique(s)
cell attachment: suitable, cell culture | mammalian: suitable
General description
Research Category: Stem Cell Research
Research Sub Category: Adhesion (CAMs)
Research Sub Category: Adhesion (CAMs)
Application
Poly-L-Ornithine Solution (0.01%) has been used to coat:
- glass-bottom plates for cell plating in a research study investigating the localization of cMET in MDA-MB-468 cells using confocal microscopy
- culture dishes for adult human neural stem cells (ahNSCs) primary culture[1]
- 6-well plates for induced pluripotent stem cells (iPSCs) culture and neuronal differentiation[2]
Physical form
Product is presented in sterile water at 0.01% w/v (0.1mg/ml). Product is filtered through a 0.2 micron filter before freezing.
Preparation Note
1. Thaw Poly-L-Ornithine solution at room temperature.
2. Dilute Poly-L-Ornithine solution to the desired concentration in sterile water.
3. Fully coat the cell culture surface with diluted Poly-L-Ornithine solution. Use 5 mL volume for 6-cm plates and 10 mL volume for 10-cm plates and T75 flasks.
4. Allow the cell culture vessel to sit at room temperature overnight.
5. Aspirate the Poly-L-Ornithine solution the following day and rinse the vessel with sterile water followed by coating with desired ECM protein. Optimal coating concentrations must be determined by the end user. Typical coating concentrations range from 10ug/ml to 100ug/ml based on cell type and application.
2. Dilute Poly-L-Ornithine solution to the desired concentration in sterile water.
3. Fully coat the cell culture surface with diluted Poly-L-Ornithine solution. Use 5 mL volume for 6-cm plates and 10 mL volume for 10-cm plates and T75 flasks.
4. Allow the cell culture vessel to sit at room temperature overnight.
5. Aspirate the Poly-L-Ornithine solution the following day and rinse the vessel with sterile water followed by coating with desired ECM protein. Optimal coating concentrations must be determined by the end user. Typical coating concentrations range from 10ug/ml to 100ug/ml based on cell type and application.
Store at -20°C . Avoid multiple freeze thaw cycles to maintain product integrity.
Analysis Note
Solution is clear with no particulates present. Solution is suitable for cell culture applications.
Legal Information
NOVASEPTUM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Cet article | |||
|---|---|---|---|
| technique(s) cell attachment: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell attachment: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| biological source synthetic | biological source synthetic | biological source synthetic | biological source - |
| concentration 0.01 % (w/v), 0.1 mg/mL | concentration 0.01% | concentration 0.01 % (w/v), 0.1 mg/mL | concentration - |
| form liquid | form solution | form liquid | form liquid |
| mol wt Mw 78000 Da | mol wt 30,000-70,000 | mol wt Mw 84000 Da | mol wt - |
| manufacturer/tradename NovaSeptum® | manufacturer/tradename - | manufacturer/tradename NovaSeptum® | manufacturer/tradename - |
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 1
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Could I dilute Poly-L-Ornithine solution (A-004-C) to the desired concentration in PBS rather then sterile water?
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