598502

Thioredoxin Reductase, Yeast, Recombinant, E. coli

• Synonyme(s) : ryNTR
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.54

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 100
• Pureté: ≥95% (SDS-PAGE)
• Forme: lyophilized solid
• Activité spécifique: ≥5 units/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Solubilité: aqueous buffer, pH 7.0: soluble
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

Recombinant, yeast thioredoxin reductase expressed in E. coli. Yeast thioredoxin reductase is a flavoprotein that has no selenocysteine (SeCys). It catalyzes the NADPH-dependent reduction of thioredoxin as well as other endogenous and exogenous proteins. Plays an important role in many aspects of cell function, including protection against oxidative stress/injury, cell growth and transformation, and the recycling of ascorbate from its oxidized form.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will cause an absorbance change of 1 at 412 nm using 200 µM NADPH and 5 mM DTNB, per min at 25°C, pH 7.0.

Forme physique

Lyophilized from 150 mM NaCl, 20 mM phosphate buffer, pH 7.4.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for 1 month at 4°C or 3 months at -70°C.

Autres remarques

Mustacich, D. and Powis, G. 2000. Biochem. J.346, 1.
Wang, P.F., et al. 1998. Biochemistry37, 16378.
Holmgren, A., 1979. J. Biol. Chem. 254, 9627.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable