535142

Protease Inhibitor Cocktail I

Animal-Free, lyophilized, for the inhibition of serine, esterases, cysteine, trypsin-like and metalloproteases. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

• Synonyme(s) : Animal-Free Protease Inhibitor Mix, Protease inhibitor
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• product name: Protease Inhibitor Cocktail Set I, Animal-Free, The Protease Inhibitor Cocktail Set I, Animal-Free controls the activity of Protease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.
• Forme: lyophilized
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated (hygroscopic)
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

A cocktail of five protease inhibitors for the inhibition of a broad range of proteases and esterases. Each vial, when reconstituted with 1 ml H₂O, yields a 100X stock solution. When diluted to 1X the cocktail contains 500 µM AEBSF, HCl (Cat. No. 101500), 150 nM Aprotinin (Cat. No. 616371), 1 µM E-64 (Cat. No. 324890), 0.5 mM EDTA, Disodium Salt and 1 µM Leupeptin Hemisulfate (Cat. No. 108975). Available as 1 ml or as a set of 10 x 1 ml.

Application

Protease Inhibitor Cocktail Set I, Animal-Free has been used:

• as a supplement in cell lysis buffer to extract proteins from non-small-cell lung carcinoma (NSCLC) cell lines
• in NP-40 buffer to lyse the cells for sodium dodecyl sulfate-12% polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting
• as a component in lysis buffer to lyse transfected Huh7 cells and to wash the pellets to detect hepatitis B virus (HBV) C protein phosphorylation in the cell

Actions biochimiques/physiologiques

Protease inhibitor cocktails and specific protease inhibitors are valuable resources for scientists involved in proteomic studies, such as protein expression, characterization, biomarker discovery, post-translational modification mapping, protein profiling, and quantitative protein measurement. By adding a protease inhibitor cocktail or specific inhibitor, the integrity of proteins is preserved for subsequent analysis and further sample characterization.

Avertissement

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Reconstitute each vial in 1 ml H₂O to yield a 100X stock solution.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS08,GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H314,H332,H373
• Conseils de prudence: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P314
• Classification des risques: Acute Tox. 4 Inhalation - Eye Dam. 1 - Skin Corr. 1A - STOT RE 2 Inhalation
• Organes cibles: Respiratory Tract
• Code de la classe de stockage: 8A - Combustible corrosive hazardous materials
• Classe de danger pour l'eau (WGK): WGK 3