5.06029

LipidGreen2

• Synonyme(s) : LipidGreen2
• Formule empirique (notation de Hill): C₂₂H₂₉NO₄
• Poids moléculaire : 371.47
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.21

Propriétés

• Pureté: ≥97% (HPLC)
• Niveau de qualité: 100
• Forme: oil
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Couleur: orange-yellow
• Solubilité: DMSO: 100 mg/mL
• Température de stockage: 2-8°C

Description

Description générale

A cell-permeable N-dimethylallylated 3-hydroxyindole based compound that is shown to selectively stain neutral lipids, but not phospholipids, in various cells lines with bright fluorescence and with minimal non-specific background. Generates stronger staining with lipid droplets (~10 µM) when compared to Nile Red. Shown to be more effective than BODIPY^® 493/503 in assessing hepatotoxicity of several known compounds using HepG2 cells. Also suitable for staining fat deposits in developing zebrafish embryos. Can be used in conjunction with LipidToxt red dye for multicolor imaging of neutral lipids.

A cell-permeable N-dimethylallylated 3-hydroxyindole based compound that is shown to selectively stain neutral lipids, but not phospholipids, in various cells lines with bright fluorescence and with minimal non-specific background. Generates stronger staining with lipid droplets (~10 µM) when compared to Nile Red. Shown to be more effective than BODIPY^® 493/503 in assessing hepatotoxicity of several known compounds using HepG2 cells. Also suitable for staining fat deposits in developing zebrafish embryos. Can be used in conjunction with LipidToxt red dye for multicolor imaging of neutral lipids.

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Actions biochimiques/physiologiques

Cell permeable: yes

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Autres remarques

Chun, H., et al, 2013, Mol Biosys.9, 630.

Informations légales

BODIPY is a registered trademark of Life Technologies

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable