416154

Isoginkgetin

≥98% (HPLC), solid, pre-mRNA splicing inhibitor, Calbiochem^®

• Synonyme(s) : Pre-mRNA Splicing Inhibitor, Isoginkgetin, 8-(5-(5,7-Dihydroxy-4-oxo-4H-chromen-2-yl)-2-methoxyphenyl)-5,7-dihydroxy-2-(4-methoxyphenyl)-4H-chromen-4-one
• Formule empirique (notation de Hill) : C₃₂H₂₂O₁₀
• Numéro CAS: 548-19-6
• Poids moléculaire : 566.51
• UNSPSC Code: 12352200
• NACRES: NA.77
• MDL number: MFCD00597035
• Assay: ≥98% (HPLC)
• Form: solid
• Storage condition: OK to freeze, protect from light

Propriétés

• Nom du produit: Pre-mRNA Splicing Inhibitor, Isoginkgetin, The Pre-mRNA Splicing Inhibitor, Isoginkgetin, also referenced under CAS 548-19-6, blocks the spliceosome-meidated splicing process.
• Quality Segment: 100
• assay: ≥98% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: yellow
• solubility: DMSO: 100 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: [o]1c2c([c](cc1c6ccc(cc6)OC)=O)c(cc(c2c3c(ccc(c3)c4[o]c5c([c](c4)=O)c(cc(c5)O)O)OC)O)O
• InChI: 1S/C32H22O10/c1-39-18-6-3-15(4-7-18)26-14-24(38)31-22(36)12-21(35)29(32(31)42-26)19-9-16(5-8-25(19)40-2)27-13-23(37)30-20(34)10-17(33)11-28(30)41-27/h3-14,33-36H,1-2H3
• InChI key: HUOOMAOYXQFIDQ-UHFFFAOYSA-N

Description

General description

A cell-permeable, naturally isolated Ginkgo biloba biflavanoid that acts as a general pre-mRNA splicing inhibitor (IC_max = 50 µM in splicing assays using HeLa nuclear extract) by blocking the spliceosome-meidated splicing process at the prespliceosome/A complex stage. Isoginkgetin treatment is shown to result in growth arrest in HEK293-derived cultures in a reversible manner without affecting cell viability (33 µM for 24 h).

A naturally isolated Ginkgo biloba biflavanoid that acts as a cell-permeable, reversible inhibitor against spliceosome-mediated pre-mRNA splicing (IC₅₀ and IC_max = 30 and 50 µM, respectively, in splicing assays using HeLa nuclear extract) by preventing the prespliceosome A complex from recruiting the U4/U5/U6 tri-snRNP (stable nuclear RNA and its associated proteins) to form B complex. Isoginkgetin treatment is shown to result in growth arrest in HEK293-derived cultures in a reversible manner without affecting cell viability (33 µM for 24 h).

Packaging

Packaged under inert gas

Other Notes

Kim, H.Y., et al. 2008. Arch. Pharm. Res.31, 265.
O′Brien, K., et al. 2008. J. Biol. Chem.283, 33147.
Yoon, S,O., et al. 2006. Mol. Cancer Ther.5, 2666.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable