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Merck
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Key Documents

393207-M

Millipore

Anti-HNE-Michael Adducts, Reduced Rabbit pAb

liquid, Calbiochem®

Synonyme(s) :

Anti-4-Hydroxy-2-noneal Michael Adducts, Reduced

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About This Item

Code UNSPSC :
12352203

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

serum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

liquid

Ne contient pas

preservative

Réactivité de l'espèce (prédite par homologie)

all

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles

Isotype

IgG

Température de stockage

−70°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Anti-HNE-Michael Adducts, Reduced, rabbit polyclonal, specifically recognizes chemically reduced amino acid-(4)-HNE adducts. It is validated for use in ELISA, WB & IHC (frozen & paraffin sections).
Rabbit polyclonal antibody supplied as diluted serum. Recognizes, specifically, chemically reduced amino acid-(4)-HNE adducts.
Recognizes chemically reduced amino acid-(4)-HNE adducts. Does not recognize non-reduced adducts or reduced modifications arising from malondialdehyde (MDA) and structurally-related alkenes such as trans-2-noneal.
The CNS is enriched in polyunsaturated fatty acids and is therefore particularly vulnerable to lipid peroxidation. 4-Hydroxy-2-noneal (HNE) is one of the major lipid peroxidation products with cytotoxic and mutagenic activity. It reacts with protein residues such as histidine to generate stable Michael adducts. HNE has been shown to inhibit neurite outgrowth and disrupt microtubules and tubulin modification, suggesting it may play a role in Alzheimer′s disease.

Spécificité

A broad range of species

Immunogène

HNE-treated KLH in which 1:1 amino acid-HNE Michael adducts were chemically reduced

Application

ELISA (1:500-1:1000)

Immunoblotting (1:1000-1:4000)

Frozen Sections (1:50-1:500)

Paraffin Sections (1:50-1:500)

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Serum diluted in 50% glycerol, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Autres remarques

Does not bind non-reduced adducts and reduced modifications arising from malondialdehyde (MDA) or structurally-related alkenals such as trans-2-noneal. Does not cross-react with non-reduced amino acid-HNE Michael adducts. Antibody binding requires the presence of the 4-hydroxyl group. Variables associated with assay conditions will dictate the optimal working dilution.
Toyokuni, S., et al. 2000. Antioxid. Redox. Signal2, 681.
Neely, M.D., et al. 1999. J. Neurochem.72, 2323.
Uchida, K., et al. 1995. Biochem. Biophys. Res. Commun.212, 1068.
Uchida, K., et al. 1995. Arch. Biochem. Biophys.324, 241.
Uchida, K., et al. 1994. Biochemistry33, 12487.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

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