343222

Biotin, Fluorescein Conjugate

• NACRES: NA.41
• UNSPSC Code: 41116133

Propriétés

• biological source: (Synthetic chemical)
• Quality Segment: 100
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• dilution: (EIA (1:1,000); Tissue Staining (1:50 - 1:100))
• color: orange
• solubility: DMF: 5 mg/mL, DMSO: 5 mg/mL, methanol: 5 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

It can be used for the detection of avidin and streptavidin.

Application

EIA (1:1,000)

Tissue Staining (1:50 - 1:100)

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Other Notes

Gruber, H.J., et al. 1998. Biochim. Biophys. Acta1381, 203

Make a stock solution by dissolving in DMF (DMSO and methanol should also work). Suggested concentration for stock solution is 5 mg/ml. Dilute stock solution with appropriate buffer for assay just prior to use. For tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50 - 1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1,000. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• Que se passe-t-il ?: WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable