324716

O-Glycosidase

Hydrolyzes O-linked glycans, suitable for mass spectrometry, recombinant, expressed in E. coli

• Synonyme(s) : Endo-α-N-acetylgalactosaminidase, Streptococcus pneumoniae, Recombinant, E. coli, O-Glycopeptide endo-D-galactosyl-N-acetyl-α-galactosaminohydrolase, O-Glycosidase
• Numéro CAS: 59793-96-3
• Numéro CE : 3.2.1.97 (BRENDA, IUBMB)
• MDL number: MFCD00007030
• NACRES: NA.42
• UNSPSC Code: 12352202
• Specific activity: ≥1 units/mL, ≥10 units/mg protein
• Recombinant: expressed in E. coli

Propriétés

• Nom du produit: Endo-α-N-acetylgalactosaminidase, Streptococcus pneumoniae, Recombinant, E. coli, Endo-α-N-acetylgalactosaminidase, Streptococcus pneumoniae, Recombinant, E. coli, CAS 59793-96-3, catalyzes the hydrolysis of the unsubstituted Galβ1,3GalNAc core disaccharide attached to Ser or Thr.
• recombinant: expressed in E. coli
• Quality Segment: 100
• conjugate: (O-linked)
• form: liquid
• specific activity: ≥1 units/mL, ≥10 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• technique(s): protein mass spectrometry: suitable
• foreign activity: N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidase, proteases, none detected
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Note: 1 mU = 1 milliunit.

Recombinant, Streptococcus pneumoniae Endo-α-N-acetylgalactosaminidase expressed in E. coli. Catalyzes the hydrolysis of the unsubstituted Galβ1,3GalNAc core disaccharide attached to serine or threonine residues of glycopeptides and glycoproteins to afford free oligosaccharides. For carbohydrates containing sialic acid or fucose, pretreatment with neuraminidase or fucosidase is required.

Physical form

In 50 mM sodium phosphate buffer, pH 7.5.

Other Notes

One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-2-acetamido-2-deoxy-3-O-(β-D-galactopyranosyl)-α-D-galactopyranoside per min at 37°C, pH 5.0.

Wang, A.M., et al. 1998. Mol. Genet. Metab. 65, 165.
Iwase, H., and Hotta, K. 1993. Methods Mol. Biol. 14, 151.
Fan, J.Q., et al. 1990. Agric. Biol. Chem. 54, 233.
Umemoto, J., et al. 1978. Anal. Biochem. 91, 186.
Glasgow, L.R., et al. 1977. J. Biol. Chem. 252, 8615.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Informations sur la sécurité

• Classe de stockage: 10 - Combustible liquids
• Que se passe-t-il ?: nwg