2384
Creatine Phosphokinase, Rabbit Skeletal Muscle
Synonyme(s) :
Creatine Phosphokinase, Rabbit Skeletal Muscle, rCPK
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A propos de cet article
Numéro CAS:
UNSPSC Code:
12352202
NACRES:
NA.21
MDL number:
Specific activity:
≥300 U/mg, 25 °C, 750 U/mg, 37 °C
Biological source:
rabbit skeletal muscle
Service technique
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Laissez-nous vous aiderbiological source
rabbit skeletal muscle
Quality Segment
form
lyophilized
specific activity
≥300 U/mg, 25 °C, 750 U/mg, 37 °C
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated (hygroscopic)
solubility
0.1 M imidazole buffer, pH 6.6: soluble, 0.5% NaHCO3, pH 9.0: soluble
foreign activity
ATPase, hexokinase, myokinase <0.001%
shipped in
ambient
storage temp.
−20°C
General description
Native creatine phosphokinase from rabbit muscle. Catalyzes the reversible conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.
Preparation Note
Following reconstitution in 0.5% NaHCO₃, refrigerate (4°C). Do not freeze or shake, as SH groups may be oxidized and form linkages between protein molecules, resulting in precipitation, turbidity, and loss of activity. NaHCO₃ stock solutions are stable for 2-3 days at 4°C. Following reconstitution in 0.1 M imidazole buffer, aliquot and freeze (-20°C). Imidazole stock solutions are stable for up to 4 weeks at -20°C. Avoid freeze/thaw cycles, as 20-30% of activity will be lost with each freeze/thaw cycle.
Other Notes
Bessman, S.P. 1987. Anal. Biochem.161, 519.
One unit is defined as the amount of enzyme that will catalyze the conversion of 1.0 µmol creatine phosphate per min at 25°C or 37°C, pH 6.8.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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