208731

Calpain Substrate II, Fluorogenic

Cyclic fluorogenic substrate for the quantitative determination of calpain -1 and -2.

• Synonyme(s) : Calpain Substrate II, Fluorogenic, Suc-LY-AMC
• Formule empirique (notation de Hill) : C₂₉H₃₃N₃O₈
• Numéro CAS: 94367-20-1
• Poids moléculaire : 551.59
• UNSPSC Code: 12352200
• NACRES: NA.77
• MDL number: MFCD00057900
• Assay: ≥95% (TLC)
• Form: solid
• Storage condition: OK to freeze, desiccated, protect from light

Propriétés

• Quality Segment: 100
• assay: ≥95% (TLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated, protect from light
• color: off-white
• solubility: DMSO: soluble
• fluorescence: λ_ex ~380 nm, λ_em ~460 nm
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: N([C@@H](C(CC)C)C(=O)NC(Cc3ccc(cc3)O)C(=O)Nc1cc2c(cc1)C(=CC(=O)O2)C)C(=O)CCC(=O)O
• InChI: 1S/C29H33N3O8/c1-4-16(2)27(32-24(34)11-12-25(35)36)29(39)31-22(14-18-5-8-20(33)9-6-18)28(38)30-19-7-10-21-17(3)13-26(37)40-23(21)15-19/h5-10,13,15-16,22,27,33H,4,11-12,14H2,1-3H3,(H,30,38)(H,31,39)(H,32,34)(H,35,36)/t16?,22?,27-/m0/s1
• InChI key: VSWNAJAOJZXWBM-FTJGSZSVSA-N

Description

General description

Cyclic fluorogenic substrate for the quantitative determination of calpain -1 and -2. Also suitable for measuring the peptidase activity of the 20S proteasome. Typical concentrations range from 10 - 100 µM, depending on assay conditions.

Fluorogenic substrate for the quantitative determination of porcine calpain I and II activity. Also serves as a substrate for other proteases including proteasome. (excitation wavelength: 380 nm; emission wavelength: 460 nm)

Biochem/physiol Actions

Cell permeable: no

Primary Target
Calpain -1 and -2

Product does not compete with ATP.

Reversible: no

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Seol, J.H., et al. 1989. FEBS Lett. 247, 197.
Woo, K.M., et al. 1989. J. Biol. Chem. 264, 2088.
Sasaki, T., et al. 1984. J. Biol. Chem.259, 12489.

Suc-Leu-Tyr-AMC

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable