189724

Ascorbate Oxidase, Cucurbita sp.

Native ascorbate oxidase from Cucurbita species. Catalyzes the oxidation of ascorbioxidation of ascorbic acid to dehydroascorbic acid. Suitable for eliminating interference of ascorbic acid in clinical assays and for determination of ascorbic acid.

• Synonyme(s) : Ascorbate Oxidase, Cucurbita sp.
• Numéro CAS: 9029-44-1
• Numéro CE : 1.10.3.3 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.54
• MDL number: MFCD00130560
• Specific activity: ≥1500 U/mg, ≥300 units/mg material

Propriétés

• Quality Segment: 100
• form: lyophilized
• specific activity: ≥1500 U/mg, ≥300 units/mg material
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated (hygroscopic)
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Native ascorbate oxidase from Cucurbita species. A multi-copper-containing oxidase that catalyzes oxidation of ascorbic acid to dehydroascorbic acid. AO catalyses the four-electron reduction of dioxygen to water with concominant one-electron oxidation of the reducing organic substrate. Suitable for eliminating interference of ascorbic acid in clinical assays and for determination of ascorbic acid. Inhibited by azide, dithiocarbamate, and thiocyanate.

Preparation Note

Following reconstitution refrigerate (4°C). This Stock solutions are stable for up to 3 weeks at 4°C.

Other Notes

One unit is defined as the amount of enzyme that will oxidize 1.0 µmol ascorbic acid per min at 37°C, pH 5.6.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

enzyme will denature below pH 4.0.

Informations sur la sécurité

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable