17-641

ChIPAb+ REST - ChIP Validated Antibody and Primer Set

• UNSPSC Code: 12352203
• NACRES: NA.52
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: rat, human, rabbit, mouse
• Application: ChIP, WB
• Citations: -

Propriétés

• antibody form: affinity purified immunoglobulin
• Quality Segment: 100
• clone: polyclonal
• species reactivity: rat, human, rabbit, mouse
• technique(s): ChIP: suitable, western blot: suitable
• UniProt accession no.: Q13127

Description

General description

120 kDa predicted, 200 kDa observed

Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.

SUBUNIT STRUCTURE: Interacts with SIN3A, SIN3B and RCOR1.

SUBCELLULAR LOCATION: NucleusProbable.

TISSUE SPECIFICITY: Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.



SEQUENCE CAUTION: The sequence AAA98503.1 differs from that shown. Reason: Frameshift at positions 189, 212 and 1087.

The sequence AAH38985.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence.

The REST purified antibody is made against a GST fusion protein corresponding to amino acids 801-1097 of human REST.

Application

Épitope : a.a. 801-1097

Biochem/physiol Actions

Recognizes REST, Mr 120-200 kDa.

Packaging

25 expériences par jeu. Utilisation recommandée : μ

Physical form

Anti-REST (rabbit polyclonal IgG). μμ

μμ

μμ

TGG GTT TTA GGA CCA GGA TG

Preparation Note

Stable for 1 year at -20°C from date of receipt. Recommandations relatives à la manipulation du produit : dès la première décongélation, et avant le retrait du bouchon, centrifuger le flacon et mélanger délicatement la solution. Diviser en aliquotes dans des microtubes à centrifuger et les stocker à -20 °C. Éviter les cycles de congélation/décongélation répétés, qui peuvent détériorer les IgG et nuire aux performances du produit.

Analysis Note

Successful immunoprecipitation of REST-associated DNA fragments was verified by qPCR using Control Primers.

Other Notes

Human, mouse, rat. Not tested in other species.

Includes negative control rabbit IgG antibody and control primers specific for the RE1 site of human Synapsin.

Disclaimer

Sauf indication contraire dans notre catalogue ou toute autre documentation associée au(x) produit(s), nos produits sont uniquement destinés à la recherche et ne sauraient être utilisés à d'autres fins, ce qui inclut, sans s'y limiter, les utilisations commerciales non autorisées, les utilisations diagnostiques in vitro, les utilisations thérapeutiques ex vivo ou in vivo, ou tout type de consommation ou d'application chez l'être humain ou chez l'animal.

Informations sur la sécurité

• Classe de stockage: 10 - Combustible liquids
• wgk: WGK 2