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Merck

12-303

Jurkat Cell Lysate

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A propos de cet article

UNSPSC Code:
41105330
NACRES:
NA.41
eCl@ss:
32011807
Technique(s):
western blot: suitable
Species reactivity:
-
Application:
WB
Citations:
8
Service technique
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biological source

human

Quality Level

form

liquid

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

shipped in

dry ice

Biochem/physiol Actions

Jurkat

Physical form

Modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM NaCl, 1mM EDTA, 1mM PMSF, 1mg/ml aprotinin, 1mg/ml leupeptin, 1mg/ml pepstatin, 1mM Na3VO4, 1mM NaF) and diluted with non-reducing sample buffer (31mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue).

Preparation Note

6 months at -20°C

Analysis Note

Routinely evaluated as a positive control for some of Upstates antibodies.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


pictograms

Exclamation mark

Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2



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Xavier Le Guezennec et al.
Molecular and cellular biology, 26(3), 843-851 (2006-01-24)
The human genome contains a number of methyl CpG binding proteins that translate DNA methylation into a physiological response. To gain insight into the function of MBD2 and MBD3, we first applied protein tagging and mass spectrometry. We show that
Peixuan Zhu et al.
Leukemia & lymphoma, 54(3), 579-586 (2012-07-31)
Overexpression of zeta-chain-associated protein 70 (ZAP-70) was recently recognized as an independent prognostic marker for the aggressive form of chronic lymphocytic leukemia (CLL). The objective of this study was to demonstrate the feasibility and implementation of quantitative detection of ZAP-70
Sonia D'Souza et al.
Blood, 118(26), 6871-6880 (2011-11-02)
Protracted inhibition of osteoblast (OB) differentiation characterizes multiple myeloma (MM) bone disease and persists even when patients are in long-term remission. However, the underlying pathophysiology for this prolonged OB suppression is unknown. Therefore, we developed a mouse MM model in



Numéro d'article de commerce international

RéférenceGTIN
12-30308436037125348