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Buffer tablets pH 6.8

for preparing buffer solution acc. to WEISE for the staining of blood smears

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A propos de cet article

NACRES:
NA.21
UNSPSC Code:
41116121
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Quality Segment

form

tablet

IVD

for in vitro diagnostic use

pH

6.8 (20 °C in H2O)

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

General description

Buffer tablets pH 6.8 - for preparing buffer solution according to Weise for the staining of blood smears, are used to prepare buffer solutions according to Weise and pH-buffered staining solutions. Phosphate buffer solutions are used in hematology, for the dilution of staining solutions and for the rinsing steps in the staining process. The buffer tablets according to Weise will produce a buffered solution (pH 6.4/pH 6.8/pH 7.2) that stabilizes the staining result and gives reproducible staining results. Buffer solution according to Weise pH 6.4 results in bright-orange stained erythrocytes and differentiated stained cells with nuclei, whereby the erythrocytes are stained orange when using pH 6.8. Buffer solution acc. to Weise pH 7.2 produces grayish stained erythrocytes and intense red-violet stained cells with nuclei.
100 tablets are sufficient for 100L buffer solution, each produced solution can be stable up to 4 weeks. Buffer tablets according to Weise are IVD products and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

pH-value (1 tablet in 1 l of water): 6.75 - 6.95
Crushing strength: ≥ 50 N
Disintegration time: ≤ 20 min
Suitability for blood-smear staining: conforms


Classe de stockage

13 - Non Combustible Solids

Que se passe-t-il ?

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable



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Questions

1–2 of 2 Questions  
  1. How much water to add to one tablet to make approximately 1 gallon of buffer solution?

    1 answer
    1. Each tablet should be reconstituted in 1 liter of distilled water. 4 tablets can be used to prepared 4 liters of buffer. 4 liters is the equivalent of 3.8 gallons.

      Helpful?

  2. Is this item a direct replacement for Gurr's Buffer pH 6.8 from other suppliers? Both products are phosphate-based with a pH of 6.8, but there is limited information available on the formulation. Intended application is Wright Giemsa staining for cytogenetics analysis.

    1 answer
    1. The Gurr, Weise, and Sorensen's phosphate buffers are generally considered interchangeable for use in hematology; however, without knowing specific formulation information we cannot definitively say this product is a direct replacement.

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