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1.09033

Schiff′s reagent

for microscopy

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A propos de cet article

NACRES:
NA.41
UNSPSC Code:
41116121
Form:
liquid
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Quality Segment

form

liquid

IVD

for in vitro diagnostic use

pH

2.1-2.5 (25 °C in H2O)

density

1.01 g/cm3 at 20 °C

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

General description

Schiff‘s reagent -for microscopy for the periodic acid Schiff, or PAS reaction, is a ready-to-use solution used for the detection of mucopolysaccharides, glycogen, muco- and glycoproteins, glyco-, phospholipids, basal membranes and collagen. The PAS (periodic acid Schiff) reaction is one of the most frequently used chemical methods for the detection of aldehyde and mucosubstances in histology. In the PAS reaction, the histological specimen material is first treated with periodic acid, resulting in the oxidation of the 1,2-glycols into aldehyde groups. The addition of Schiff′s reagent (fuchsin-sulfuric acid) in the second step causes the aldehydes to react to form a brilliant red color. In the end result, the PAS reaction yields a specific color reaction with unsubstituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins, and glyco- and phospholipids. The PAS reaction can be further combined with the Alcian blue staining method to detect mucosubstances (glycosaminoglycans). 500 ml are sufficient for approx. 2500 - 3000 stainings. It is registered as IVD and CE certified product and can be used for clinical diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Application

For microscopy. Ready-to-use staining solution for human-medical cell diagnosis.


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Classe de stockage

12 - Non Combustible Liquids

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WGK 1



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Questions

  1. Which recipie was used to prepare this soluzione?

    1 answer
    1. This staining reagent is a ready-to-use preparation containing the following ingredients:
      Pararosaniline (Basic Fuchsin): 0.91 g/L
      Sodium Sulfite (Na2SO3): > 8.0 g/L
      Hydrochloric acid (HCl): 0.4 %
      Please see the link below to access the Instructions for Use:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/181/959/109033ug-ms.pdf

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