07-214

Anti-dimethyl-Histone H3 (Arg17) Antibody

Upstate^®, from rabbit

• Synonyme(s) : H3R17me2, Histone H3 (di methyl R17), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human, vertebrates
• Application: ELISA, ICC, WB, inhibition assay
• Citations: 39

Propriétés

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, vertebrates
• manufacturer/tradename: Upstate^®
• technique(s): ELISA: suitable, immunocytochemistry: suitable, inhibition assay: suitable (peptide), western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_003484
• UniProt accession no.: Q16695
• shipped in: dry ice
• target post-translational modification: trimethylation (Arg17)
• Gene Information: human ... H3C1(8350)

Description

General description

L'histone H3.1t (UniProt : Q16695; also known as H3/t, H3tm, H3/g) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) chez l'être humain. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. The H3.1 and H3.3 complexes also possess distinct histone chaperones, CAF-1 and HIRA, which play important role in mediating DNA-synthesis-dependent and -independent nucleosome assembly. It has been reported that Histone H3.1 serves as the canonical histone, which is incorporated during DNA replication, whereas H3.3 acts as the replacement histone that can be incorporated outside of S-phase during chromatin-disrupting processes like transcription. Histone H 3.1 is a core component of nucleosome that is present only in mammals and is usually enriched in acetylation of Lysine 15 and demethylation of lysine 10 (HeK9Me2). It is expressed during S phase, then expression decreases significantly as cell division slows down during the process of differentiation. Histone H 3.1 expression is shown to be replication dependent. It s presence at the site of UV-induced DNA damage has also been reported. It has also been shown that H3.1/H4 tetramers do not split and remain intact during replication dependent deposition of H3.1 variant. Dimethylation of H3 on Arginine 17 has been linked to gene activation and activation of this modification is linked to cell fate regulation and increases the pluripotency potential of stem cells. In pancreatic beta cells expression of Protein arginine methyltransferase 4 (PRMT4) and demethylation of Arginine 17 is shown to increase under high glucose conditions. (réf. : Kim, JK et al. (2015). J. Mol. Endocrinol. 54(3); 315-24).

Poids réel (observé) : env. 17 kDa ; poids théorique (calculé) : 15,51 kDa. Des bandes non caractérisées peuvent être observées avec certains lysats.

Immunogen

BSA-conjugated linear peptide corresponding to 11 amino acids from the N-terminal region of human Histone H3 dimethylated on Arginine 17 (methionine removed).

Application

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Domaine de recherche
Épigénétique et fonction nucléaire

Peptide Inhibition Assay Analysis/Western Blotting: A 1:7,500 dilution from a representative lot detected dimethyl-Histone H3 (Arg17) in Histone H3 with CARM1.

un précédent lot de cet anticorps a été utilisé en ELISA. At dilutions greater than 1:2000 this antibody is specific for peptides containing dimethylated Arg17.


An independent laboratory showed positive immunostaining for CARM-1 specific methylation of Histone H3 in 3134 cells.

Sous-domaine de recherche
Histones

Biochem/physiol Actions

Broad cross-reactivity expected based on sequence homology among species.

This rabbit polyclonal antibody specifically detects human Histone H3 dimethylated on Arginine 17.

Physical form

Anticorps polyclonal de lapin purifié, dans un tampon à base de Tris-glycine 0,1 M (pH 7,4) et de NaCl 150 mM contenant 0,05 % d'azoture de sodium.

Chromatographie d'immuno-affinité

Preparation Note

Stable à -20 °C pendant 1 an à compter de la date de réception. Recommandations relatives à la manipulation du produit : dès réception, et avant le retrait du bouchon, centrifuger le flacon et mélanger délicatement la solution. Répartir en aliquotes dans des microtubes à centrifuger et conserver ces derniers à -20 °C. Éviter les congélations/décongélations répétées, qui peuvent détériorer les IgG et nuire aux performances du produit.

Analysis Note

A 1:7,500 dilution of this antibody detected dimethyl-Histone H3 (Arg17) in Histone H3 treated with Histone-arginine methyltransferase (CARM1).

Other Notes

Concentration : pour connaître la concentration spécifique du lot, voir le certificat d'analyse.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Sauf indication contraire dans notre catalogue ou toute autre documentation associée au(x) produit(s), nos produits sont uniquement destinés à la recherche et ne sauraient être utilisés à d'autres fins, ce qui inclut, sans s'y limiter, les utilisations commerciales non autorisées, les utilisations diagnostiques in vitro, les utilisations thérapeutiques ex vivo ou in vivo, ou tout type de consommation ou d'application chez l'être humain ou chez l'animal.

Informations sur la sécurité

• Classe de stockage: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable