05-637

Anti-Bmi-1 Antibody, clone F6

clone F6, Upstate^®, from mouse

• Synonyme(s) : Polycomb group RING finger protein 4, RING finger protein 51
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: F6, monoclonal
• Espèces réactives: rat, human, mouse, rabbit
• Conditionnement: antibody small pack of 25 μg
• Fabricant/nom de marque: Upstate^®
• Technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG1
• Numéro d'accès NCBI: NM_005180
• Numéro d'accès UniProt: P25916; P35226
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... BMI1(648)

Description

Description générale

The Bmi1 protein is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors.
Several lines of evidence implicate Bmi1 in tumorigenesis. Firstly, Bmi1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. Secondly, Bmi1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene leads to neoplastic transformation. Thirdly, the BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. Fourthly, the transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires Bmi1. Indeed, based on a list of genes differentially expressed in a mouse model of metastatic prostate cancer on a wild-type and bmi1-deficient genetic background a gene signature was recently identified that may predict response to therapy and survival in multiple types of cancer.

Spécificité

Recognizes Bmi-1 (triplet).

Immunogène

Immunized with recombinant Bmi-1 protein corresponding to residues 1-202 of mouse Bmi-1.

Application

Anti-Bmi-1 Antibody, clone F6 is a Mouse Monoclonal Antibody for detection of Bmi-1 also known as Polycomb group RING finger protein 4, RING finger protein 51 and has been validated in ICC, IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Qualité

routinely evaluated by immunoblot on RIPA lysates from U2OS cells.

Description de la cible

40-44 kDa

Forme physique

Format: Purified

Immunoaffinity Purified immunoglobulin in Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH 7.4.

Protein G Purified

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
U2OS whole cell lysate, K-562 nuclear extract, 0-4 hour embryos or 12-24 hour embryos

Autres remarques

feature_concentration_valuePlease refer to lot specific datasheet.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.