05-338

Anti-Fas Antibody

UPSTATE^®, mouse monoclonal, ZB4

• Synonyme(s) : APO-1 cell surface antigen, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, CD95 antigen, FASLG receptor, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, apoptosis antigen 1, tumor necrosis factor receptor superfamily member 6, tu
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: ZB4, monoclonal
• Species reactivity: human
• Application: FACS, Neutral, WB
• Citations: 47

Propriétés

• Nom du produit: Anti-Fas Antibody (human, neutralizing), clone ZB4, clone ZB4, Upstate^®, from mouse
• biological source: mouse
• Quality Segment: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: ZB4, monoclonal
• species reactivity: human
• manufacturer/tradename: Upstate^®
• technique(s): flow cytometry: suitable, neutralization: suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_152877
• UniProt accession no.: P25445
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... FAS(355)

Description

General description

45 kDa

Fas (also known as Apo-1 and CD95) is a 40-50 kDa cell surface glycoprotein and a member of the TNF receptor family. Fas is an apotosis signalling molecule that forms the death inducing signalling complex (DISC) upon binding by the Fas ligand trimer on an adjacent cell. The Fas receptor also trimerizes, after which it is internalized and can then bind to the adaptor molecule FADD. FADD facilitates binding of caspase-8. which is activated to induce caspase-8 mediated apoptosis. Fas also plays a role in T cell mediated cytotoxicity and T cell development. Fas is found in the thymus, liver, heart, and ovary, where it is expressed on a minority of resting T and B cells and monocytes. The antagonistic ZB4 clone anti-Fas monoclonal has been used to block the apoptosis-inducing activity of the Anti-Fas CH-11 clone in multiple publications.

This product does not induce apoptosis in cell culture.

Immunogen

Recombinant human Fas.

Application

&&

Cytométrie en flux : μ Western blotting : μ

Domaine de recherche
Apoptose et cancer

Sous-domaine de recherche
Apoptose - Autre

Biochem/physiol Actions

This antibody does not recognize TNF and mouse Fas.

This antibody recognizes the human cell surface antigen Fas expressed in various human cells, including myeloid cells, T lympho-blastoid cells and diploid fibroblasts. It does not induce apoptosis in cell culture.

Physical form

Ammonium sulfate and purified mouse monoclonal IgG1 in PBS containing 50% glycerol. À conserver à -20 °C.

Format : Produit purifié

Produit purifié sur protéine A

Preparation Note

Stable pendant 1 an à compter de la date de réception.

Analysis Note

10-500 ng/mL of this antibody was incubated for one hour with human Fas-transfected cells and inhibited the anti-Fas (human, activating), clone CH11 (Catalog # 05-201). Cell viability was assessed using a WST-1 assay

Other Notes

Concentration : pour connaître la concentration spécifique du lot, voir le certificat d'analyse.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Sauf indication contraire dans notre catalogue ou toute autre documentation associée au(x) produit(s), nos produits sont uniquement destinés à la recherche et ne sauraient être utilisés à d'autres fins, ce qui inclut, sans s'y limiter, les utilisations commerciales non autorisées, les utilisations diagnostiques in vitro, les utilisations thérapeutiques ex vivo ou in vivo, ou tout type de consommation ou d'application chez l'être humain ou chez l'animal.

Informations sur la sécurité

• Classe de stockage: 10 - Combustible liquids
• wgk: WGK 2