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Merck

05-233

Anti-Nitrotyrosine Antibody, clone 1A6

clone 1A6, Upstate®, from mouse

Synonyme(s) :

Anti-nitrotyrosine antibody

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
1A6, monoclonal
Species reactivity:
vertebrates
Application:
ICC, IHC, IP, WB
Citations:
160
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biological source

mouse

Quality Segment

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1A6, monoclonal

species reactivity

vertebrates

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG2bκ

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... NOS1(4842)

General description

Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Previous studies have shown that nitrotyrosine has been found in inflammatory conditions such as atherosclerotic plaques and rheumatoid arthritis, among others
Predicted MW for nitrated-BSA: 66 kDa

Application

Anti-Nitrotyrosine Antibody, clone 1A6 detects level of Nitrotyrosine & has been published & validated for use in IC, IH, IP & WB.

Physical form

Format: Purified
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 0.014M phosphate buffer, pH 7.6, 0.175M NaCl, 0.07% sodium azide, and 30% glycerol.

Analysis Note

Control
Immunohistochemistry: Human Alzheimer disease brain sections (Methacarn fixed), mouse liver
Immunoblotting: Nitrated-BSA
Indirect ELISA: nitrated-BSA
Indirect immunofluorescence: A549 cells metabolically labeled with 3-nitrotyrosine
routinely evaluated on peroxynitrite-treated A431 cells; no reaction was noted with non-nitrated proteins

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany


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