05-1951

Anti-trimethyl Histone H3 (Lys27) Antibody, clone 18E9.1

clone 18E9.1, from mouse

• Synonyme(s) : H3K27me3, Histone H3 (tri methyl K27), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Type de produit anticorps: primary antibodies
• Clone: 18E9.1, monoclonal
• Espèces réactives: human, mouse
• Technique(s): dot blot: suitable; immunocytochemistry: suitable; western blot: suitable
• Isotype: IgG2bκ
• Numéro d'accès NCBI: NP_002098
• Numéro d'accès UniProt: P84243
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: trimethylation (Lys27)
• Informations sur le gène: human ... H3C1(8350) ; mouse ... H3C1(360198)

Description

Description générale

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Spécificité

This antibody recognizes Histone H3 trimethylated at Lys27.

Immunogène

Epitope: Trimethylated Lys27

Linear peptide corresponding to human Histone H3 trimethylated at Lys27.

Application

Anti-trimethyl Histone H3 (Lys27) Antibody, clone 18E9.1 is a mouse monoclonal antibody for detection of trimethyl Histone H3 (Lys27) also known as H3K27me3, Histone H3 (tri methyl K27) & has been validated in WB, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Western Blot Analysis: A 1:25,000 dilution from a representative lot detected Histone H3 in 10 µg of modified and nonmodified peptides.

Western Blot (Snap i.d.) Analysis: A 1:25,000 dilution from a representative lot detected Histone H3 in 10 µg of HeLa acid extract.

Immunocytochemistry Analysis: A 1:125 dilution from a representative lot detected Histone H3 in A431 and NIH/3T3 cells.

Dot Blot Analysis: Histone peptides with various modifications (see table) were transferred to
PVDF membrane and probed with Anti-Trimethyl Histone H3 (Lys4) (1:100 dilution). Proteins were visualized using a Donkey Anti-rabbit IgG conjugated to HRP and a chemiluminescence detection systems.

Qualité

Evaluated by Western Blot in HeLa acid extract.

Western Blot Analysis: A 1:50,000 dilution of this antibody detected Histone H3 in 10 µg of HeLa acid extract.

Description de la cible

~17 kDa observed

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
HeLa acid extract

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable