04-150

Anti-AIRE Antibody, clone 5H12

clone 5H12, from rat

• Synonyme(s) : Autoimmune regulator, Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein homolog, APECED protein homolog, AIRE
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rat
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 5H12, monoclonal
• Espèces réactives: mouse
• Technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG2cκ
• Numéro d'accès NCBI: NP_001258478
• Numéro d'accès UniProt: Q9Z0E3
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Aire(11634)

Description

Description générale

AIRE (Autoimmune Regulator) is a human gene that is expressed primarily in the thymus. It causes transcription of a wide selection of organ-specific genes, reducing the threat of autoimmunity occurring by allowing the elimination of autoreactive T cells via negative selection. Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendo-crinopathy-candidiasis-ectodermal dystrophy (APECED). Disruption of AIRE results in the development of a range of autoimmune diseases, the most common clinical conditions in the syndrome are hypoparathyroidism, primary adrenocortical failure and chronic mucocutaneous candidiasis. Aire functions through initiating the transcription of a diverse set of self-antigens, such as insulin, in the thymus. This expression then allows maturing thymocytes to become tolerant towards peripheral organs, thereby suppressing autoimmune disease. Aire is atranscriptional regulator protein that binds to DNA as dimer and tetramer, but not as a monomer. It forms nuclear bodies and interacts with the transcriptional coactivator CBP (CREBBP). At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD).

Immunogène

Linear peptide corresponding to the C-terminus of mouse AIRE.

Application

Detect AIRE using this rat monoclonal antibody, Anti-AIRE Antibody, clone 5H12 validated for use in Flow Cytometry, western blotting, ICC & IHC.

Research Category
Inflammation & Immunology

Research Sub Category
Inflammation & Autoimmune Mechanisms

Western Blotting Analysis: A representative lot from an independent laboratory detected AIRE in AIRE transfected COS cells (Hubert, F. X., et al. (2008). J Immunol. 180(6):3824-3832.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected AIRE in mouse thymus sections (Hubert, F. X., et al. (2008). J Immunol. 180(6):3824-3832.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected AIRE in mouse thymic epithelial cells (Guerau-de-Arellano, M., et al. (2009). J Exp Med. 206(6):1245-1252.).

Qualité

Evaluated by Flow Cytometry in mouse primary splenocytes.

Flow Cytometry Analysis: 4 µg of this antibody detected AIRE in 1X10E6 mouse primary splenocytes.

Description de la cible

60 kDa calculated. Many isoforms are known to exist.

Forme physique

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2cκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable