03-903
GFP, FLAG-tagged SNRNP70 mRNA
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A propos de cet article
NACRES:
NA.52
UNSPSC Code:
41116158
Service technique
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1 ea of 50 μg
Quality Segment
manufacturer/tradename
Millipore
growth mode
N/A
technique(s)
RIP: suitable, cell culture | mammalian: suitable, flow cytometry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
shipped in
dry ice
storage temp.
-65 to -96°C
Application
Both mRNAs and non-coding RNAs interact with RNA-binding proteins and regulate critical cellular processes and their dysregulation contributes to multiple diseases. Thus, analyzing RNA-protein interactions are critical for understanding the mechanism of diseases and designing clinical strategies.
RNA-binding protein immunoprecipitation (RIP) has been used to interrogate RNA binding proteins (RBP) associated with specific RNA molecules. However, the experiments are not feasible because of the unavailability of antibodies for the particular RBP or low expression of the RBP. To overcome these issues epitope tags have been used for RIP experiments.
RNA-binding protein immunoprecipitation (RIP) has been used to interrogate RNA binding proteins (RBP) associated with specific RNA molecules. However, the experiments are not feasible because of the unavailability of antibodies for the particular RBP or low expression of the RBP. To overcome these issues epitope tags have been used for RIP experiments.
Features and Benefits
The GFP, FLAG-tagged SNRNP70 mRNA was developed to enable to perform control RIP experiments with RIPAb+ FLAG (Cat. No. 03-901) or RIPAb+ GFP (Cat. No. 03-902) RIP validated antibody and primer kits, and Magna RIP kit (Cat. # 17-700).
Preparation Note
Store at -80ºC
Other Notes
The high amount of GFP-FLAG-tagged SNRNP70 protein is expressed immediately after transfection to cells and the well-known specific interaction of SNRNP70 and U1snRNA can be used for control RIP experiments with anti-GFP or anti-FLAG antibodies.
Disclaimer
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Classe de stockage
12 - Non Combustible Liquids
Que se passe-t-il ?
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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