L040030

Cap A

configured for ABI

• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.21

Propriétés

• Type: for DNA synthesis
• Niveau de qualité: 200
• Gamme de produits: Proligo Reagents
• Forme: liquid
• Durée de conservation: 365 days
• Concentration: 97.0-119.0 g/L (Acetic Anhydride content, titration)
• Couleur: clear colorless, to brownish
• Compatibilité: configured for ABI
• Température de stockage: 2-8°C

Description

Description générale

Cap A solution contains acetic anhydrideanhydride, lutidine and tetrahydrofuran (THF). It is used after the phosphoramidite reaction/coupling step. The solution is configured for ABI.

Actions biochimiques/physiologiques

Cap A solution aborts chains bearing unreacted 5′ hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′ hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Notes préparatoires

Tetrahydrofurane / Lutidine / Acetic Anhydride 8/1/1 (v/v/v)

Informations sur la sécurité

• Pictogrammes: GHS02,GHS06,GHS08,GHS05
• Mention d'avertissement: Danger
• Mentions de danger: H225,H302,H315,H318,H331,H335,H336,H351
• Conseils de prudence: P210 - P280 - P301 + P312 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313
• Classification des risques: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Risques supp: EUH019
• Code de la classe de stockage: 3 - Flammable liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): 6.1 °F
• Point d'éclair (°C): -14.4 °C