G005827
LC/MS (TOF) Analysis of Warfarin Enantiomers in Plasma on Astec® CHIROBIOTIC® V after SPE using HybridSPE®-Phospholipid
application for HPLC
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About This Item
technique(s)
HPLC: suitable
test parameters
sample/matrix: Rat plasma stabilized with K2EDTA was acquired from Lampire Biological Laboratories, (Pipersville PA). (Plasma was spiked directly from stock standard to a level of 100 ng/mL in 3:1 (plasma:1% formic acid acetonitrile).)
SPE tube/cartridge: Pk1 50 mg/96-Well HybridSPE-Phospholipid Plate (575656-U)
condition: Apply 100 μL of plasma to plate, followed by 300 μL of 1% formic acid acetonitrile. Agitate via vortex for 4 min.
elution: Place on vacuum manifold and apply 10” Hg vacuum for 4 minutes. Collect filtrate and analyze directly.
column: CHIROBIOTIC V,10 cm x 4.6 mm I.D., 5 μm particles (11022AST)
mobile phase: [A] 0.1% formic acid water; [B] acetonitrile, (75:25, A:B)
flow rate: 1 mL/min
column temp.: 35 °C
detector: ESI+, 100-1000 m/z
injection: 2 μL
suitability
application for HPLC
application(s)
clinical
pharmaceutical (small molecule)
Analysis Note
Legal Information
Analyte
SPE tube or plate
analytical column
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