ZRB1118
Anti-p-CREB (Ser133) Antibody, clone 1H13 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Cyclic AMP-responsive element-binding protein 1
About This Item
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1H13, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
General description
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Immunogen
Application
Evaluated by Western Blotting in lysate from Phorbol-12-Myristate-13-Acetate (PMA) treated HeLa cells.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected phospho-CREB (Ser133) in lysate from HeLa cells serum starved overnight and treated with Phorbol-12-Myristate-13-Acetate (PMA; 200 nM), but not in untreated cells.
Tested applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected phospho-CREB (Ser133) in human stomach cancer tissue sections.
Affinity Binding Assay:: A representative lot of this antibody bound phospho-CREB (Ser133) peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Peptide Inhibition Assay: Target band detection in HeLa cell lysate was prevented by preblocking of a representative lot with the phopho-CREB (Ser133) peptide, but not the corresponding non-phosphopeptide.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p-CREB (Ser133) in HeLa cells treated with Phorbol-12-Myristate-13-Acetate (PMA).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Physical form
Reconstitution
Storage and Stability
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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