U6253

Ubiquitin from bovine erythrocytes

BioUltra, ≥98% (SDS-PAGE), essentially salt-free, lyophilized powder

• Synonym(s): ATP-dependent proteolytic factor, Ub
• CAS Number: 79586-22-4
• MDL number: MFCD00082121
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: bovine erythrocytes
• Quality Level: 200
• product line: BioUltra
• Assay: ≥98% (SDS-PAGE)
• form: essentially salt-free, lyophilized powder
• storage condition: (Tightly closed. Dry)
• technique(s): western blot: suitable
• impurities: salt, essentially free
• solubility: water: 1 mg/mL, clear, colorless
• UniProt accession no.: P0CG48
• storage temp.: 2-8°C
• Gene Information: bovine ... LOC(101902760)

Description

General description

Research area: Cancer

Ubiquitin is a highly conserved regulatory protein. It is found in all eukaryotic cells and is virtually identical across all forms of life including yeast, humans, and plants. ubiquitin structure contains seven Lys residues and an N-terminus, all of which are target sites for ubiquitination.

This product is from bovine red blood cells. It is not a recombinant product. The production process is considered proprietary, however it includes heat treatment as well as purification by chromatography and dialysis.

Application

Ubiquitin from bovine erythrocytes has been used to study the role of exogenous ubiquitin in chronic β-adrenergic receptor (β-AR)-stimulated myocardial remodeling. It has also been used to test the inhibitor of nuclear factor kappa-B kinase subunit beta (IKKβ) ubiquitylation.

Ubiquitin from bovine erythrocytes can be used for in vitro ubiquitinylation assay. The product can also be used as a marker in western blotting.

Biochem/physiol Actions

Ubiquitination is a post-translational modification process where ubiquitin-protein is attached to a substrate protein. Ubiquitination plays a vital role in the regulation of cellular signaling in various biological processes such as apoptosis, protein processing, immune response, and DNA repair. Ubiquitination mediates protein degradation via the ubiquitin-proteasome pathway. Ubiquitination is implicated in various cellular signaling pathways. Polyubiquitination modulates the signal activation of NF-κ-B inhibitor alpha (IkB-α) in the inflammatory signaling pathway. Elevated levels of ubiquitin have been observed in various diseases such as parasitic and allergic diseases, alcoholic liver disease, type 2 diabetes, β2-microglobulin amyloidosis, and chronic hemodialysis.

Preparation Note

Ubiquitin from bovine erythrocytes can dissolved in water at 1 mg/ml to yield a clear, colorless solution.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves