T8778

Trehalase from porcine kidney

buffered aqueous glycerol solution, ≥1.0 units/mg protein

• Synonym(s): α,α-Trehalose glucohydrolase
• CAS Number: 9025-52-9
• Enzyme Commission number: 3.2.1.28 (BRENDA, IUBMB)
• MDL number: MFCD00132462
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: buffered aqueous glycerol solution
• Quality Level: 200
• specific activity: ≥1.0 units/mg protein
• concentration: 0.5-10.0 mg/mL protein (BCA)
• storage temp.: −20°C

Description

General description

Trehalase is a glycosidase that occurs in many forms and is found in all tissues. It belongs to the glycoside hydrolase 37 (GH37) family of the carbohydrate-active enzyme (CAZy) classification.

Trehalose is the main haemolymph sugar in most insects and is potentially a prime target for an invading pathogenic fungus.

Application

Trehalase has been used:

• in a study to assess changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves
• in a study to investigate growth arrest by trehalose-6-phosphate
• to quantify the intracellular trehalose in Lactobacillus casei cells

Biochem/physiol Actions

Trehalase catalyzes the hydrolysis of trehalose to produce two glucose units. It mediates flight metabolism, molting induced chitin production and tolerance towards cold temperature.

Unit Definition

One unit will convert 1.0 μmole of trehalose to 2.0 μmoles of glucose per min at pH 5.7 at 37 °C (liberated glucose determined at pH 7.5).

Physical form

Solution in 50% glycerol containing 1% Triton^™ X-100 and 25 mM potassium phosphate, pH 6.5

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable