Skip to Content
Merck
All Photos(1)

Key Documents

SRP3159

Sigma-Aldrich

SPARC/Osteonectin human

recombinant, expressed in CHO cells, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture

Synonym(s):

BM-40, ON, Secreted protein acidic and rich in cysteine

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

Pricing and availability is not currently available.

biological source

human

recombinant

expressed in CHO cells

Assay

≥97% (HPLC)
≥97% (SDS-PAGE)

form

lyophilized

potency

0.5-0.7 μg/mL

mol wt

43.7 kDa

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
490156604666604712
isotopic purity

≥99 atom % 13C

isotopic purity

99 atom % 13C

isotopic purity

99 atom % 13C

isotopic purity

99 atom % 13C

assay

99% (CP)

assay

-

assay

-

assay

99% (CP)

form

solid

form

solid

form

solid

form

solid

mp

222 °C (dec.) (lit.)

mp

222 °C (dec.) (lit.)

mp

240 °C (dec.) (lit.)

mp

222 °C (dec.) (lit.)

optical activity

[α]25/D +14.6°, c = 2 in 1 M HCl

optical activity

[α]25/D +14.6°, c = 2 in 1 M HCl

optical activity

-

optical activity

[α]25/D +14.6, c = 2 in 1 M HCl

General description

SPARC (secreted protein acidic and cysteine rich) is a multifunctional Ca2+-binding glycoprotein that belongs to the matricellular protein family, and is one of the eight members of the SPARC family. In adult animals, SPARC protein is generally expressed in remodeling tissues, such as bone, mucosa of the gut, and healing wounds. It shows predominant expression in tumors and in disorders linked with fibrosis. It is composed of three domains, which include a novel follistatin-like module, the acidic N-terminal (NT) domain and an extracellular calcium-binding EC module containing two EF-hand motifs. It is also known as osteonectin, and has a molecular weight of 32kDa. The gene is localized to human chromosome 5q33.1, and the encoded protein is a single polypeptide composed of 285 amino acids.[1][2]

Biochem/physiol Actions

SPARC (secreted protein acidic and cysteine rich) binds to the structural matrix protein, such ascollagen and vitronectin, and thus, regulates cell interaction with extracellular matrix (ECM). It also has counter-adhesive effects on cells by negatively influencing focal adhesions. It arrests cells in the G1 phase of cell cycle, and hence, suppresses cell proliferation. SPARC protein regulates various growth factors, such as platelet-derived growth factor (PDGF), fibroblast growth factor 2 (FGF), and vascular endothelial growth factor (VEGF).[1] In human cancers, this protein controls the activity and microenvironment of cancer cells, cell growth, apoptosis, adhesion, migration and invasion. It also controls the ECM and the activity of matrix metalloproteinases. In pancreatic cancer patients, the expression of this protein, especially in the stroma, might be linked with unfavorable prognosis.[2] In mechanically-ventilated premature infants, SPARC levels in tracheal aspirate help determine the development of bronchopulmonary dysplasia (BPD) or death.[3]

Sequence

APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVI

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.6.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Need A Sample COA?

This is a sample Certificate of Analysis (COA) and may not represent a recently manufactured lot of this specific product.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

SPARC, a matricellular protein: at the crossroads of cell-matrix.
Brekken RA, Sage EH, et al.
Matrix Biology, 19(7), 569-580 (2000)
R V Talmage et al.
Journal of musculoskeletal & neuronal interactions, 6(4), 402-407 (2006-12-23)
This report summarizes the evidence that the control of the concentration of free calcium ions in body fluids is centered at mineralized bone surfaces. This process involves an increase in the solubility of bone mineral produced by the non-collagenous proteins
Tracheal Aspirate Levels of the Matricellular Protein SPARC Predict Development of Bronchopulmonary Dysplasia.
Popova AP, et al.
PLoS ONE, 10(12) (2015)
Prognostic Value of SPARC in Patients with Pancreatic Cancer: A Systematic Review and Meta-Analysis.
Han W, et al.
PLoS ONE, 11(1) (2016)
Susana Cruz-Neves et al.
Journal of biomedical materials research. Part A, 105(7), 2035-2046 (2017-04-04)
Prostate cancer (PCa) is the second leading cause of death among men in Europe and U.S. The metastatic dissemination pattern of PCa is unique, developing bone metastasis as the only site of progression, consequently with a prognosis very poor. The

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service