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SRP0249

Sigma-Aldrich

PLK2 Active human

recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)

Synonym(s):

Polo-like kinase 2, SNK, Serum-inducible kinase

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

Pricing and availability is not currently available.

biological source

human

recombinant

expressed in baculovirus infected insect cells

Assay

≥60% (SDS-PAGE)

form

aqueous solution

specific activity

≥3.6 pmol/min-μg

mol wt

calculated mol wt 107 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

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This Item
490156604666604712
isotopic purity

≥99 atom % 13C

isotopic purity

99 atom % 13C

isotopic purity

99 atom % 13C

isotopic purity

99 atom % 13C

assay

99% (CP)

assay

-

assay

-

assay

99% (CP)

form

solid

form

solid

form

solid

form

solid

mp

222 °C (dec.) (lit.)

mp

222 °C (dec.) (lit.)

mp

240 °C (dec.) (lit.)

mp

222 °C (dec.) (lit.)

optical activity

[α]25/D +14.6°, c = 2 in 1 M HCl

optical activity

[α]25/D +14.6°, c = 2 in 1 M HCl

optical activity

-

optical activity

[α]25/D +14.6, c = 2 in 1 M HCl

General description

Human Plk2 (GenBank Accession No. NM_006622), full length, with N-terminal GST tag, expressed in a baculovirus infected Sf9 cell expression system. The theoretical MW is 107 kDa.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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M V Ramana Reddy et al.
Bioorganic & medicinal chemistry, 24(4), 521-544 (2016-01-15)
Several families of protein kinases have been shown to play a critical role in the regulation of cell cycle progression, particularly progression through mitosis. These kinase families include the Aurora kinases, the Mps1 gene product and the Polo Like family

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